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KH-type splicing regulatory protein controls colorectal cancer cell growth and modulates the tumor microenvironment

ABSTRACT: KH-type splicing regulatory protein controls colorectal cancer cell growth and modulates the tumor microenvironment

PROVIDER: PRJNA445699 | ENA |

REPOSITORIES: ENA

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KH-type splicing regulatory protein controls colorectal cancer cell growth and modulates the tumor microenvironment

Project description:KH-type splicing regulatory protein (KHSRP) is a multifunctional nucleic acid binding protein. KHSRP regulates transcription, mRNA decay and translation, and miRNA biogenesis that influence distinct functions associated with cancer cell biology, such as inflammation and cell-fate determination. Our study uncovered novel mechanistic-based data on the tumor-promoting effects of KHSRP in colorectal cancer. We used microarrays to detail changes in gene expression upon specific knockdown of KHSRP in SW480 cells

2019-05-08 | GSE112329 | GEO

KH-Type Splicing Regulatory Protein Controls Colorectal Cancer Cell Growth and Modulates the Tumor Microenvironment.

Project description:KH-type splicing regulatory protein (KHSRP) is a multifunctional nucleic acid binding protein implicated in key aspects of cancer cell biology: inflammation and cell-fate determination. However, the role KHSRP plays in colorectal cancer (CRC) tumorigenesis remains largely unknown. Using a combination of in silico analysis of large data sets, ex vivo analysis of protein expression in patients, and mechanistic studies using in vitro models of CRC, we investigated the oncogenic role of KHSRP. We demonstrated KHSRP expression in the epithelial and stromal compartments of both primary and metastatic tumors. Elevated expression was found in tumor versus matched normal tissue, and these findings were validated in larger independent cohorts in silico. KHSRP expression was a prognostic indicator of worse overall survival (hazard ratio, 3.74; 95% CI, 1.43-22.97; P = 0.0138). Mechanistic data in CRC cell line models supported a role of KHSRP in driving epithelial cell proliferation in both a primary and metastatic setting, through control of the G1/S transition. In addition, KHSRP promoted a proangiogenic extracellular environment by regulating the secretion of oncogenic proteins involved in diverse cellular processes, such as migration and response to cellular stress. Our study provides novel mechanistic insight into the tumor-promoting effects of KHSRP in CRC.

| S-EPMC6892187 | biostudies-literature

KH-type splicing regulatory protein mediates changes in colorectal cancer cell secretome

Project description:KH-type splicing regulatory protein (KHSRP) is a multifunctional nucleic acid binding protein. KHSRP regulates transcription, mRNA decay and translation, and miRNA biogenesis that influence distinct functions associated with cancer cell biology, such as inflammation and cell-fate determination. However, the role KHSRP plays in colorectal cancer (CRC) tumorigenesis is largely unknown. Our study uncovered novel mechanistic-based data on the tumor-promoting effects of KHSRP in CRC, including the control by KHSRP of protein secreted by CRC epithelial cells, influencing the tumor-promoting microenvironment.

2018-03-23 | MSV000082206 | MassIVE

KH-type splicing regulatory protein (KHSRP) is involved in esophageal squamous cell carcinoma progression through regulating a subset of microRNAs [miRNA]

Project description:KH-type splicing regulatory protein (KHSRP) is a multifunctional RNA-binding protein (RBP), which is involved in many post-transcriptional aspects of RNA metabolism including microRNA (miRNA) biogenesis, affects distinct cell functions in different tissues and can impact on various pathological conditions. However, the function and underlying mechanisms of KHSRP in esophageal squamous cell carcinoma (ESCC) remain largely unknown. In the present study, we uncover a novel oncogenic function of KHSRP in esophageal tumorigenesis, and implicate its use as a marker for prognostic evaluation and as a putative therapeutic target in ESCC.

2018-02-22 | GSE99423 | GEO

KH-type splicing regulatory protein (KHSRP) is involved in esophageal squamous cell carcinoma progression through regulating a subset of microRNAs [mRNA]

2018-02-22 | GSE99422 | GEO

PRMT5 Modulates Splicing in Hematopoietic Stem Cells

Project description:PRMT5 Modulates Splicing in Hematopoietic Stem Cells

| PRJNA479853 | ENA

Estrogen signaling modulates the colon microenvironment during obesity

Project description:Estrogen signaling modulates the colon microenvironment during obesity

| PRJNA630214 | ENA

HRPK-1, a conserved KH-domain protein, modulates microRNA activity during Caenorhabditis elegans development

Project description:HRPK-1, a conserved KH-domain protein, modulates microRNA activity during Caenorhabditis elegans development

| PRJNA573615 | ENA

An SWAP1-SFPS-RRC1 splicing factor complex modulates pre-mRNA splicing to promote photomorphogenesis in Arabidopsis

Project description:To investigate the molecular and physiological functions of SWAP1 in light signaling. We show that SWAP1 modulates global gene expression and alternative splicing both under dark and red light treated conditions

2022-11-09 | GSE214299 | GEO

Transcriptome-wide effect profile of HuR inhibitor KH-3

Project description:The purpose of this study is to generate mRNA effect profle of KH-3, an inhibitor of RNA-binding protein HuR, in human breast cancer cell line MDA-MB-231 cells by RNA sequencing. Total RNA from cells treated with DMSO or KH-3 was collected for library prepareation and deep sequencing, in duplicate, using Illumina Hiseq 2500 system. About 30 million sequence reads per sample were mapped against the transcript databases annotated in GENCODE v24 using STAR default parameters. The read counts for each transcript were calculated using eXpress. The differentially expressed transcripts were detected using DESeq2 with a q-value cutoff of 0.05.

2019-04-05 | GSE129362 | GEO

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