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Genome-wide maps of chromatin remodeling factors Isw2 and Ino80 in asynchronously growing yeast cells

ABSTRACT: Genome-wide maps of chromatin remodeling factors Isw2 and Ino80 in asynchronously growing yeast cells

PROVIDER: PRJNA445972 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6908150.fastq.gz	Fastqsanger.gz
	SRR6908151.fastq.gz	Fastqsanger.gz
	SRR6908152.fastq.gz	Fastqsanger.gz
	SRR6908145.fastq.gz	Fastqsanger.gz
	SRR6908146.fastq.gz	Fastqsanger.gz

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Genome-wide maps of chromatin remodeling factors Isw2 and Ino80 in asynchronously growing yeast cells

Project description:ChIP-seq of Isw2 (subunit of Isw2 complex) and Nhp10 (subunit of Ino80 complex) was performed on two independent isolates of FLAG-tagged Isw2 or Nhp10 Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>

2018-10-30 | GSE112423 | GEO

Genome-wide maps of nucleosome positioning in asynchronously growing yeast cells

Project description:MNase-seq was performed in WT, isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: MNase_<growth condition>_<genotype-replicate>_<strain name>_<units MNase>

2018-10-30 | GSE112427 | GEO

Genome-wide maps of histone H3 in asynchronously growing yeast cells

Project description:ChIP-seq of histone H3 was performed on two biological replicates of the same W303 strain of wild-type cells and two independent isolates of each of isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>

2018-10-30 | GSE112424 | GEO

Genome-wide maps of DNA Polymerase pausing in asynchronously growing yeast cells

Project description:ChIP-seq of FLAG-tagged Pol2 (subunit of DNA Polymerase epsilon) was performed on two independent isolates of each of WT, isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>

2018-10-30 | GSE112426 | GEO

Genome-wide maps of histone H3 in asynchronously growing yeast cells

Project description:Genome-wide maps of histone H3 in asynchronously growing yeast cells

| PRJNA445976 | ENA

Genome-wide maps of nucleosome positioning in asynchronously growing yeast cells

Project description:Genome-wide maps of nucleosome positioning in asynchronously growing yeast cells

| PRJNA445977 | ENA

Genome-wide maps of DNA Polymerase pausing in asynchronously growing yeast cells

Project description:Genome-wide maps of DNA Polymerase pausing in asynchronously growing yeast cells

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Genome-wide maps of RNA Polymerase I and III in asynchronously growing yeast cells

Project description:ChIP-seq of FLAG-tagged RPA190 (subunit of RNA Polymerase I) or FLAG-tagged RPO31 (subunit of RNA Polymerase III) was performed on WT and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype>_<strain name>

2018-10-30 | GSE112425 | GEO

Genome-wide maps of RNA Polymerase I and III in asynchronously growing yeast cells

Project description:Genome-wide maps of RNA Polymerase I and III in asynchronously growing yeast cells

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Yeast replication in the presence of MMS: WT and isw2 nhp10 strains

Project description:The eukaryotic DNA replication machinery must traverse every nucleosome present in the eukaryotic genome during S phase. Since nucleosomes are generally inhibitory to DNA-dependent processes, it is thought that chromatin structure must undergo extensive reorganization to facilitate DNA synthesis. However, the identity of chromatin-remodeling factors involved in replication and how they affect DNA synthesis is largely unknown. Here we show that two ATP-dependent chromatin-remodeling complexes in Saccharomyces cerevisiae, Isw2 and Ino80, function in parallel to promote DNA replication, especially during periods of replication stress. The rate of replication-fork progression is slowed when both chromatin-remodeling pathways are compromised. Both Isw2 and Ino80 complexes are recruited to actively replicating chromatin suggesting that these chromatin-remodeling complexes act directly to promote replication-fork progression. These findings support an important role for ATP-dependent chromatin-remodeling complexes in promoting DNA replication, and define specific stages of replication that require remodeling activity for normal function. A critical part of this study was the use of microarrays to measure DNA replication progression throughout the genome during MMS treatment. These samples are from experiments where we competed newly replicated DNA against unreplicated DNA on the same microarrays. Keywords: Time course

2007-11-30 | GSE9122 | GEO

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