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Role and function of Class III LitR, a photosensor homolog from Burkholderia multivorans ATCC 17616 in the regulation of folate production.

ABSTRACT: Role and function of Class III LitR, a photosensor homolog from Burkholderia multivorans ATCC 17616 in the regulation of folate production.

PROVIDER: PRJNA447436 | ENA |

REPOSITORIES: ENA

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Role and function of Class III LitR, a photosensor homolog from Burkholderia multivorans ATCC 17616 in the regulation of folate production.

Project description:The LitR/CarH protein family is a adenosyl B12 (AdoB12)-dependent photoreceptor with DNA-binding activity and its homologs are widely distributed in the genome of diverged bacterial genera. Here, we studied a role and function of a LitR homolog from a Gram-negative soil bacteria Burkholderia multivorans, which has no AdoB12-binding domain. Transcriptome analysis revealed the existence of 19 light-induced genes (folE, cfa, litS, a Photolyase gene phrB2, cryB2) located in the flanking region of litR. Gene disruption of litR caused a constitutive expression of all light-inducible genes, while the mutation of the light-dependent expressed sigma factor litS abolished the transcription in cryB2, phrB2 operon and cfa operon, indicating that LitR and LitS play a central role in the light-inducible transcription. Gel-shift assay showed that a recombinant protein LitR specifically binds to the promoter region of litR and folE2 operon, and its ability was lowered by UV-A illumination. LitR absorbs maximally near 340 nm, and exhibited a photocyclic response and light-dependent subunit dissociation. Furthermore, the litR mutant produced a 20-fold high intracellular folate compared to the wild-type strain. Overall, the line of evidence suggest that Class III LitR light-dependently regulates the transcription of litR itself and folE2-litS operon, leading the production of folate, and then expressed RNA polymerase-LitS directs the transcription for phrB2 operon and cfa operon. Light-dependent characteristics suggests that Class III LitR in complex with a UV-A absorbing molecule has a novel light-sensing mechanism.

2018-10-09 | GSE112472 | GEO

Expression data from Burkholderia multivorans ATCC 17616 wild-type and Bmul_2557 (ldhR) gene deletion mutant

Project description:Within this work we identified and characterized gene Bmul_2557 (ldhR) of B. multivorans ATCC 17616, a bacterial species associated with chronic respiratory infections in cystic fibrosis patients. LdhR belongs to the LysR-type family of transcriptional regulators and its deletion from the B. multivorans genome affected considerably the formation of planktonic cellular aggregates and surface-attached biofilms.

2017-07-28 | GSE97006 | GEO

Burkholderia multivorans reference dataset-Experiment 3

Project description:The gene expression of the opportunictic cystic fibrosis lung pathogen Burkholderia multivorans ATCC 17616 was investigated under different growth conditions relevant for growth in the cystic fibrosis lung.

2012-05-11 | E-MEXP-3210 | biostudies-arrayexpress

Burkholderia multivorans reference dataset-Experiment 1

2012-05-10 | E-MEXP-3208 | biostudies-arrayexpress

Burkholderia multivorans reference dataset-Experiment 2

2012-05-11 | E-MEXP-3209 | biostudies-arrayexpress

Hybridization of Burkholderia multivorans D2095 and D2214 genomic DNA

Project description:Burkholderia cepacia complex (Bcc) comprises opportunistic bacteria infecting hosts such as cystic fibrosis (CF) patients. Bcc long-term infection of CF patient airways has been associated with emergence of phenotypic variation. Here we studied two Burkholderia multivorans clonal isolates (D2095 and D2214) displaying different morphotypes from a chronically infected CF patient in order to evaluate traits development during lung infection. Since the custom array described in platform GPL13356 was based on Burkholderia multivorans ATCC 17616 genome, here we performed a DNA-DNA hybridization to determine which probes of the array hybridize with our test genomes

2011-08-22 | GSE30402 | GEO

Hybridization of Burkholderia multivorans D2095 and D2214 genomic DNA

2011-08-21 | E-GEOD-30402 | biostudies-arrayexpress

Burkholderia multivorans

Project description:Expression data from Burkholderia multivorans ATCC 17616 wild-type and Bmul_2557 (ldhR) gene deletion mutant

| PRJNA380390 | ENA

Rheinheimera pacifica strain:DSM 17616

Project description:Rheinheimera pacifica DSM 17616

| PRJNA262429 | ENA

Overexpression of a histone-like nucleoid structuring protein in Burkholderia multivorans affects several traits dependent on the cell envelope

Project description:Burkholderia cepacia complex bacteria comprise opportunistic pathogens causing chronic respiratory infections in cystic fibrosis (CF) patients. These microorganisms produce exopolysaccharides which are considered virulence determinants. Here, we report the isolation of an evolved nonmucoid variant (17616nmv) after the ancestor, Burkholderia multivorans ATCC 17616, was subjected to prolonged stationary phase. Lack of exopolysaccharide cepacian biosynthesis in the variant was correlated with downregulation of bce genes expression. Furthermore, genome sequence of the variant identified the transposition of a mobile element of the IS406 family into the promoter region of an hns-like gene (Bmul_0158) encoding a histone-like nucleoid structuring protein, a known global transcriptional repressor. This IS element upregulated the expression of Bmul_0158 gene. Transcriptome analysis identified the global effects of this mutation on gene expression, with major changes in motility, pili synthesis, type VI secretion, and chromosome associated functions. Concomitant with these differences, the nonmucoid variant displays reduced adherence to a CF lung bronchial cell line, reduced surface hydrophobicity, forms smaller cellular aggregates, but has an increase in swimming and swarming motilities. Finally, bioinformatic analysis led to the identification of various genomic regions, possibly acquired by horizontal gene transfer, which were transcriptionally repressed by the increased expression of hns gene in the nonmucoid variant. Taken together, our results revealed a significant role for this H-NS protein in the regulation of B. multivorans persistence- and virulence-associated genes.

2021-04-26 | GSE162693 | GEO

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