Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression in tobacco (Nicotiana) nectaries

ABSTRACT: Gene expression in tobacco (Nicotiana) nectaries

PROVIDER: PRJNA448133 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6918750_1.fastq.gz	Fastqsanger.gz
	SRR6918750_2.fastq.gz	Fastqsanger.gz
	SRR6918760_1.fastq.gz	Fastqsanger.gz
	SRR6918760_2.fastq.gz	Fastqsanger.gz
	SRR6918770_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 162

Similar Datasets

Gene expression in tobacco (Nicotiana) nectaries

Project description:Nectaries are the glands responsible for nectar secretion. To understand the genetic programming underlying nectar production, floral nectaries of six Nicotiana species at four different time points (floral stage 6,9, and 12, a post-secretory stage) in biological triplicate were collected, with RNA being isolated and subjected to Illumina RNA-seq analysis.

2018-04-12 | GSE112526 | GEO

Nicotiana nectary development

Project description:Floral Nectaries Many plants secrete a rich floral nectar to entice visitation by insect and avian pollinators. In turn, these pollinators transfer pollen between flowers increasing plant fecundity. The nectary is the floral organ that secretes nectar into the base of the flower. The size and abundance of the ornamental tobacco nectaries (Nicotiana sp.) will permit us to isolate up to several grams of nectaries at each stage to obtain the necessary amounts of RNA for probe preparation. Our primary goals to understand the biochemistry the nectary, so that we can manipulate nectary function to increase pollinator visitation. We have previously conducted an EST study and have identified 13596 cDNAs from three different stages of nectary development (Stage 6, immature, presecretory nectaries; Stage 12, mature nectaries at floral anthesis; and nectaries, 44 hours after fertilization. In our efforts to evaluate the transcriptional program for the Nicotiana nectary we are proposing to evaluate nectary mRNAs by hybridization with the potato microarrays. We have preliminary evidence that wholesale transcriptional reprogramming (60% of the transcriptome) occurs during nectary maturation and again following fertilization. Our goal is to understand these processes at a biochemical level so that we can begin manipulating nectary function to improve nectar quality and quantity thereby increasing the attractiveness of flowers to insect pollinators. Such improvements have the potential to result in increases in insect visitation, seedset, and ultimately yield for insect pollinated crops. We are also making significant efforts to understand the restructuring of the nectary during its lifecycle. Many changes occur during nectary development and the observed transcriptional reprogramming makes sense the when these many changes are accounted for. Keywords: Loop design 30 hybs total

2007-09-01 | E-GEOD-8245 | biostudies-arrayexpress

Nicotiana nectary development

2007-09-01 | GSE8245 | GEO

Transcription profiling of Nicotiana tabacum tissues throughout the lifecycle to generate the Tobacco Expression Atlas (TobEA)

Project description:Gene expression was measured in different tissues throughout the lifecycle of the Nicotiana tabacum plant to generate the Tobacco Expression Atlas (TobEA).

2009-12-05 | E-MTAB-176 | biostudies-arrayexpress

Gene expression in squash (Cucurbita pepo) nectaries

Project description:Gene expression in squash (Cucurbita pepo) nectaries

| PRJNA437911 | ENA

Transcription profiling of Nicotiana tabacum grown in different light conditions to test the reproducibility of a custom Affymetrix microarray for tobacco

Project description:A study was carried out to compare gene expression levels between technical replicate samples in order to test the reproducibility of a custom Affymetrix microarray for Tobacco (Nicotiana tabacum).

2009-01-01 | E-MTAB-72 | biostudies-arrayexpress

Gene expression in squash (Cucurbita pepo) nectaries

Project description:Nectaries are the glands responsible for nectar secretion. To understand the genetic programming underlying nectar production, male and female squash(Cucurbita pepo) floral nectaries at four different time points (pre-secretion #1, pre-secretion #2, secretory, and post-secretory) in biological triplicate were collected, with RNA being isolated and subjected to Illumina RNA-seq analysis.

2018-03-13 | GSE111695 | GEO

Expression data from transgenic Nicotiana tabacum expressing a variant of coat protein and movement protein from Tobacco mosaic virus

Project description:Transgenic expression of viral proteins in natural host plants is a useful simplified system with the potential to understand the individual effect of each viral component. Transgenic expression of movement (MP) and a variant from coat protein (CPT42W) in tobacco, a TMV natural host, produces severe morphological changes, altered miRNAs accumulation and poor fertility. We used microarrays to characterize the gene expression changes caused by the co-expression of TMV capsid and movement proteins in Nicotiana tabacum comparing two isogenic lines MPxCPT42W and mpxcpT42W* (a line with both transgenes spontaneously silenced and with normal phenotype).

2012-12-01 | GSE37905 | GEO

Tobacco pollen sequestrome dynamics

Project description:Transcriptome profiling of four RNA fractions in six developmental stages of tobacco (Nicotiana tabacum) male gametophyte

2018-05-24 | GSE114806 | GEO

Transcriptome profiling of male gametophyte development in Nicotiana tabacum

Project description:Transcriptome profiling of three developmental stages of immature male gametophyte intobacco (Nicotiana tabacum) Total RNA isolated from tobacco microspores and early and late bicellular pollen was hybridised on Agilent Tobacco Gene Expression Microarray 4x44K in two biological replicates per sample

2014-10-16 | E-GEOD-62349 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data