Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Yap1 safeguards mouse embryonic stem cells from excessive apoptosis during differentiation

ABSTRACT: Yap1 safeguards mouse embryonic stem cells from excessive apoptosis during differentiation

PROVIDER: PRJNA448443 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8260152.fastq.gz	Fastqsanger.gz
	SRR8260153.fastq.gz	Fastqsanger.gz
	SRR8260154.fastq.gz	Fastqsanger.gz
	SRR8260155.fastq.gz	Fastqsanger.gz
	SRR8260156.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 5

Similar Datasets

Yap1 safeguards mouse embryonic stem cells from excessive apoptosis during differentiation

Project description:While Yap1, a Hippo pathway transcriptional effector, plays numerous roles in development and cancer, its functions in embryonic stem (ES) cell differentiation remain poorly characterized. It is known that approximately 30% of ES cells die after exiting self-renewal to differentiate, but upstream regulators of this process are not well known. We first observe that ES cells lacking Yap1 experience massive cell death upon the exit from self-renewal. We subsequently reveal that Yap1 contextually protects differentiating, but not self-renewing, mouse ES cells from hyperactivation of the apoptotic cascade. Mechanistically, Yap1 strongly activates anti-apoptotic genes via directly occupying their regulatory elements while mildly suppressing pro-apoptotic genes. Our demonstration of the pro-survival functions of Yap1 during ES cell differentiation expands our understanding of upstream regulators of the balance between survival and death during cell fate changes.

2018-12-20 | GSE112606 | GEO

Yap1 safeguards mouse embryonic stem cells from excessive apoptosis during differentiation.

Project description:Approximately, 30% of embryonic stem cells (ESCs) die after exiting self-renewal, but regulators of this process are not well known. Yap1 is a Hippo pathway transcriptional effector that plays numerous roles in development and cancer. However, its functions in ESC differentiation remain poorly characterized. We first reveal that ESCs lacking Yap1 experience massive cell death upon the exit from self-renewal. We subsequently show that Yap1 contextually protects differentiating, but not self-renewing, ESC from hyperactivation of the apoptotic cascade. Mechanistically, Yap1 strongly activates anti-apoptotic genes via cis-regulatory elements while mildly suppressing pro-apoptotic genes, which moderates the level of mitochondrial priming that occurs during differentiation. Individually modulating the expression of single apoptosis-related genes targeted by Yap1 is sufficient to augment or hinder survival during differentiation. Our demonstration of the context-dependent pro-survival functions of Yap1 during ESC differentiation contributes to our understanding of the balance between survival and death during cell fate changes.

| S-EPMC6307859 | biostudies-literature

Genomic integrity safeguards pluripotency in embryonic stem cells

Project description:Genomic integrity safeguards pluripotency in embryonic stem cells

| PRJNA438830 | ENA

Roles of Yap1 in mouse embryonic stem cells

Project description:We report that Yap1 is dispensable for the maintenance of ES cells while Yap1 is required for the differentiation of ES cells using global gene expression profile

2016-03-04 | GSE69669 | GEO

Effect of excessive c-MYC expression on self-replication and apoptosis of iPS cell-derived megakaryocyte progenitor cell lines

Project description:To further address the mechanism by which excessive c-MYC represses self-replication and induces apoptosis, microarray analysis of iPSC-derived megakaryocyte progenitor cell lines(MKCLs) with high or low level of c-MYC expression were examined. We established iPS cell lines integrated with DD-tagged c-MYC genes whose protein expression level could be controlled by the concentration of Shield-1. Gene expressions of iPSC-derived MKCLs with high or low levels of ectopic c-MYC were analyzed by microarray.

2014-01-17 | E-GEOD-54168 | biostudies-arrayexpress

Effect of excessive c-MYC expression on self-replication and apoptosis of iPS cell-derived megakaryocyte progenitor cell lines

2014-01-17 | GSE54168 | GEO

Transcriptional repressor FOXD3 safeguards genome integrity in embryonic stem cells

Project description:Transcriptional repressor FOXD3 safeguards genome integrity in embryonic stem cells

| PRJNA760536 | ENA

Angiotensin IV attenuates diabetic cardiomyopathy via suppressing FoxO1-induced excessive autophagy, apoptosis and fibrosis

Project description:Angiotensin IV attenuates diabetic cardiomyopathy via suppressing FoxO1-induced excessive autophagy, apoptosis and fibrosis

| PRJNA660959 | ENA

Regulation of gene expression by YAP1

Project description:In order to define YAP1-specific gene expression patterns in gastric cancer, the constitutively active mutant YAP1 (YAP1-S127A) was over-expressed in MKN45 gastric cancer cells. Defined gene expression signature was later used to stratify gastric cancer patients according to the presence of the YAP1-activated signature. Three groups of samples are included: 1. Mock control; 2. Vector control; 3. YAP-S127A expression. Gene expression profiles of YAP-S127A mutant-expressing cells were compared to that of mock and vector control. Experiments were done in MKN45 gastric cancer cells.

2013-10-01 | E-GEOD-41387 | biostudies-arrayexpress

Role of Yes-Associated Protein 1 (YAP1) in rhabdomyosarcoma

Project description:Doxycycline-inducible YAP1 S127A-driven rhabdomyosarcoma (RMS) tumors, control skeletal muscle and regressed tumors following YAP1 normalization by doxycycline withdrawal were compared to determine the YAP1-regulated gene expression profile relevant to RMS formation. To characterize the role of YAP1 in embryonal RMS at the molecular level and identify a gene signature for YAP1 activity readout, we compared the gene expression profiles of our YAP1-driven ERMS with control donor skeletal muscle (SKM) and doxycycline-withdrawn regressing tumors by microarray (doxycycline withdrawal for 3 or 6 days; OFF3 and OFF6, respectively). We next extracted a list of genes regulated by YAP1 in our YAP1-driven ERMS tumors (TUM) versus the 3 other conditions: skeletal muscle control (SKM), Doxycycline-withdrawn 3 days (OFF3) and 6 days (OFF6). The overlap between the 3 lists identified a subset of 633 common upregulated genes, named the YAP1-ERMS_UP signature, as well as 249 common downregulated genes, termed the YAP1-ERMS_DOWN signature. Proliferative pathways and transcriptional targets of E2F factors were highlighted in the YAP1-ERMS_UP genes, while muscle differentiation and trancriptional targets of myogenic factors Myod1 and Mef2 were highlighted in the YAP1-ERMS_DOWN genes. Tumor regression conditions (OFF3 days; OFF6 days) as well as control muscle (CTL) were compared with tumors at day 0 of doxycycline withdrawal (TUM). 3 samples for each conditions were used.

2014-08-12 | E-GEOD-47198 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data