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Mediator is essential for small nuclear and nucleolar RNA transcription in yeast

ABSTRACT: Mediator is essential for small nuclear and nucleolar RNA transcription in yeast

PROVIDER: PRJNA448794 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6942576_1.fastq.gz	Fastqsanger.gz
	SRR6942576_2.fastq.gz	Fastqsanger.gz
	SRR6942577_1.fastq.gz	Fastqsanger.gz
	SRR6942577_2.fastq.gz	Fastqsanger.gz
	SRR7310231_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Mediator is essential for small nuclear and nucleolar RNA transcription in yeast

Project description:Eukaryotic RNA polymerase II (RNAPII) transcribes mRNA genes and non-protein coding RNAs (ncRNAs) including small nuclear and nucleolar RNAs (sn/snoRNAs). In metazoans, RNAPII transcription of sn/snoRNAs is facilitated by a number of specialized complexes, but no such complexes have been discovered in yeast. It has been proposed that yeast sn/snoRNA promoters use the same factors as mRNA promoters, but the extent to which regulators of mRNA genes function at yeast sn/snoRNA genes is unclear. Here, we investigated a potential role for the Mediator complex, essential for mRNA gene transcription, in the transcription of sn/snoRNA genes. We found that the Mediator maps to most sn/snoRNA gene regulatory regions and that rapid depletion of the essential structural subunit Med14 strongly reduces RNAPII and TFIIB occupancy as well as nascent transcription of sn/snoRNA genes. Deletion of Med3 and Med15, subunits of the activator-interacting Mediator tail module, does not affect Mediator recruitment to or RNAPII and TFIIB occupancy of sn/snoRNA genes. Our analyses suggest that Mediator promotes PIC formation and transcription at sn/snoRNA genes, expanding the role of this critical regulator beyond its known functions in mRNA gene transcription and demonstrating further mechanistic similarity between the transcription of mRNA and sn/snoRNA genes.

2018-10-08 | GSE112721 | GEO

Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast.

Project description:Eukaryotic RNA polymerase II (RNAPII) transcribes mRNA genes and non-protein-coding RNA (ncRNA) genes, including those encoding small nuclear and nucleolar RNAs (sn/snoRNAs). In metazoans, RNAPII transcription of sn/snoRNAs is facilitated by a number of specialized complexes, but no such complexes have been discovered in yeast. It has been proposed that yeast sn/snoRNA and mRNA expression relies on a set of common factors, but the extent to which regulators of mRNA genes function at yeast sn/snoRNA genes is unclear. Here, we investigated a potential role for the Mediator complex, essential for mRNA gene transcription, in sn/snoRNA gene transcription. We found that Mediator maps to sn/snoRNA gene regulatory regions and that rapid depletion of the essential structural subunit Med14 strongly reduces RNAPII and TFIIB occupancy as well as nascent transcription of sn/snoRNA genes. Deletion of Med3 and Med15, subunits of the activator-interacting Mediator tail module, does not affect Mediator recruitment to or RNAPII and TFIIB occupancy of sn/snoRNA genes. Our analyses suggest that Mediator promotes PIC formation and transcription at sn/snoRNA genes, expanding the role of this critical regulator beyond its known functions in mRNA gene transcription and demonstrating further mechanistic similarity between the transcription of mRNA and sn/snoRNA genes.

| S-EPMC6275182 | biostudies-literature

Fission yeast essential nuclear pore protein nup211 regulates the transcriptome

Project description:Fission yeast essential nuclear pore protein nup211 regulates the transcriptome

| PRJNA892086 | ENA

Architectural Mediator subunits are differentially essential for global transcription in yeast (ChIP-seq)

Project description:Architectural Mediator subunits are differentially essential for global transcription in yeast (ChIP-seq)

| PRJNA607312 | ENA

Mapping targets for small nucleolar RNAs in yeast.

Project description:Mapping targets for small nucleolar RNAs in yeast.

| PRJNA485343 | ENA

Mapping targets for small nucleolar RNAs in yeast

Project description:Mapping targets for small nucleolar RNAs in yeast

| PRJNA472155 | ENA

Architectural Mediator subunits are differentially essential for global transcription in yeast (RNA-seq/4tU-seq)

Project description:Architectural Mediator subunits are differentially essential for global transcription in yeast (RNA-seq/4tU-seq)

| PRJNA610215 | ENA

ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast

Project description:Mediator subunits Med17 (Srb4) and Med15 (Gal11) were subjected to ChIP in wild type yeast, med18 (srb5), med20 (srb2), and med2 med3 med15 (triple mutant) yeast, all in kin28-anchor away yeast after 1 hr rapamycin treatment to evict Kin28 from the nucleus. This results in inactivation of Kin28, which reduces Mediator turnover at promoters and allows stronger Mediator ChIP signal at promoters. <br></br>Both Med17 and Med15 were tagged with myc and precipitated using the monoclonal antibody against c-myc. Please note that a specific control for rapamycin treatment was not performed in this experiment, as that has been well documented in the literature for this experiment, using what is called the anchor away method. The untagged sample is a control for non-specific immunoprecipitation.

2017-12-01 | E-MTAB-5824 | biostudies-arrayexpress

Fission yeast essential nuclear pore protein nup211 regulates the transcriptome

Project description:While nuclear pore proteins mediate nucleo-cytoplasmic transport, they can have important roles in other activities such as transcription, chromatin organization, and cell cycle regulation. The fission yeast nuclear pore protein nup211 localizes to the nuclear basket and is essential for cell viability. Nup211 is important for RNA quality control and the nuclear export of polyadenylated RNAs. Interestingly, several nup211 orthologs have also been shown to regulate transcription at the chromatin level. Although nup211 preferentially associates with heterochromatin, it is unclear whether it modulates transcription. To further investigate the functions of nup211, we constructed a strain in which nup211 expression can be reduced through the addition of thiamine to the growth medium (nup211-so) and examined how cells were affected when nup211 protein was reduced. Down-regulation of nup211 led to severe defects in cell cycle regulation, including septation and cytokinesis. Using RNA-Seq and RT-qPCR, we revealed that loss of nup211 significantly altered global gene expression. Our findings suggest a new role for nup211 in regulating the transcriptome.

2024-10-04 | GSE216106 | GEO

Functional studies of the Yeast Mediator Tail Module Subunits

Project description:The yeast Mediator complex can be divided into three modules, designated Head, Middle and Tail. Tail comprises the Med2, Med3, Med5, Med15 and Med16 protein subunits, which are all encoded by genes that are individually non-essential for viability. In cells lacking Med16, Tail is displaced from Head and Middle. However, inactivation of MED5/MED15 and MED15/MED16 are synthetically lethal, indicating that Tail performs essential functions as a separate complex even when it is not bound to Middle and Head. We have used the N-Degron method to create temperature sensitive (ts) mutants in the Mediator tail subunits Med5, Med15 and Med16 to study the immediate effects on global gene expression when each subunit is individually inactivated, and when MED5/15 or MED15/16 are inactivated together.

2013-06-07 | GSE47712 | GEO

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