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Effects of Hnf4a deletion in the nephron lineage during kidney development

ABSTRACT: Effects of Hnf4a deletion in the nephron lineage during kidney development

PROVIDER: PRJNA449100 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6956270.fastq.gz	Fastqsanger.gz
	SRR6956267.fastq.gz	Fastqsanger.gz
	SRR6956268.fastq.gz	Fastqsanger.gz
	SRR6956269.fastq.gz	Fastqsanger.gz

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Effects of Hnf4a deletion in the nephron lineage during kidney development

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Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids

Project description:Nephron progenitor cells (NPCs) self-renew and differentiate into nephrons, the functional units of the kidney. Here we report manipulation of p38 and YAP activity creates a synthetic niche that allows the long-term clonal expansion of primary mouse and human NPCs, and induced NPCs (iNPCs) from human pluripotent stem cells. Cultured iNPCs resemble closely primary human NPCs, generating nephron organoids with abundant distal convoluted tubule cells, which are not observed in published kidney organoids. The synthetic niche reprograms differentiated nephron cells into NPC state, recapitulating the plasticity of developing nephron in vivo. Scalability and ease of genome-editing in the cultured NPCs allow for genome-wide CRISPR screening, identi-fying novel genes associated with kidney development and disease. A rapid, efficient, and scala-ble organoid model for polycystic kidney disease was derived directly from genome-edited NPCs, and validated in drug screen. These technological platforms have broad applications to kidney development, disease, plasticity, and regeneration.

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Transcriptional profiles of kidney upon HNF4A loss in mice by RNA-seq

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