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Differentially expressed genes after miRNA or siRNA transfection in human cancer cell lines (SK-MES, MDA-MB-231, MKN and 786-O)

ABSTRACT: Differentially expressed genes after miRNA or siRNA transfection in human cancer cell lines (SK-MES, MDA-MB-231, MKN and 786-O)

PROVIDER: PRJNA449920 | ENA |

REPOSITORIES: ENA

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Project description:786-0 is a cell line derived from a clear cell renal carcinoma. Previous studies have shown that the 786-O cell line harbors an inactivating mutation in the von-Hippel Lindau (VHL) gene. Mutations in the VHL gene occur in the majority of sporadic clear cell renal cell. To determine how inactivation of the VHL affects cellular functions, we created a derivative of 786-0, which we call 786-VHL in which a functional allele of VHL has been introduced back into the 786-O cell line. The renal cell carcinoma cell line 786-0, which harbors a mutated allele of VHL, was compared to a cell line derived from 786-0, termed 786-VHL, that contains a functional allele of VHL. Genes whose expression characteristics were dependent on functional VHL were identified.

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