Project description:The soil bacterium Flavobacterium johnsoniae was grown on agar plates with or without pectin,bacteria were harvested, lysed, and subjected to LC-MS/MS analysis
Project description:Clinical Flavobacterium columnare ATCC 49512 was grown on Flavobacterium columnare growth medium (FCGM). Bacteria from four colonies at mid-exponential phase were harvested, total proteins were isolated, and identified using 2-DE MALDI TOF/TOF MS and 2-D LC ESI MS/MS analyses. The MS/MS spectra for all peptides were analyzed using sequest algorithm