Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide BRD4, PolII and p65 occupancy as well as chromatin modifications in bone marrow derived macrophages from BRD4 conditional KO mice

ABSTRACT: Genome-wide BRD4, PolII and p65 occupancy as well as chromatin modifications in bone marrow derived macrophages from BRD4 conditional KO mice

PROVIDER: PRJNA450452 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7012170.fastq.gz	Fastqsanger.gz
	SRR7012180.fastq.gz	Fastqsanger.gz
	SRR7012190.fastq.gz	Fastqsanger.gz
	SRR7012200.fastq.gz	Fastqsanger.gz
	SRR7012171.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 40

Similar Datasets

Genome-wide BRD4, PolII and p65 occupancy as well as chromatin modifications in bone marrow derived macrophages from BRD4 conditional KO mice

Project description:We are reporting here genome wide distribution of BRD4 in bone marrow derived macrophages at steady state level and in reponse to LPS stimulation. In both condition, BRD4 distribution was found to be widespread in intragenic as well as intergenic regions with enrichment specifically near TSS. Characteristically BRD4 signal were highly enriched on enhancers of some active genes and hence were classified as BRD4 rich super-enhancers (>12kb long stretches). Super-enhancers are formed, in some instances by redistribution of BRD4 on LPS stimulated genes. BRD4 binding well correlated PolII binding at the chromatin acetylated at H3K27, H3K9 and H4tetraK. Interestingly many LPS induced BRD4 independent genes, despite BRD4 loss in the KO, maintained PolII binding at the chromatin enriched with acetylated lysines at H3K27, H3K9 and H4tetraK. Additionally among some BRD4 independent LPS inducible genes we detected stronger p65 enrichment in the KO. In contrast, P65 binding was not detected in BRD4 dependent genes. These observations provided evidence of plasticity of epigenetic changes in retaining inflammatory reponses in macrophages.

2019-02-21 | GSE113226 | GEO

ChIP-seq analysis of BRD4, MED1 and P65 in SCC1 cells after JQ1 treatment

Project description:To explore genome-wide alteration of BRD4, MED1, p65 and H3K27Ac during BET inhibition, we performed chromatin immunoprecipitation sequencing (ChIP-seq) of SCC1 cells to examine genome-wide recruitment of the MED1, BRD4, p65 and H3K27ac following JQ1 treatment. BET inhibition by JQ1 led to dramatically loss of the recruitment of MED1, BRD4 and p65 at a cohort of key oncogenes associate with tumorigenesis and metastasis. Suggesting BET inhibition is effective strategy to suppress the tumorigenesis and metastasis of head and neck squamous cell carcinoma.

2021-05-05 | GSE131710 | GEO

PolII occupancy

Project description:Chromatin plays roles in processes governed by different time scales. To assay the dynamic behaviour of chromatin in living cells, we used genomic tiling arrays to measure histone H3 turnover in G1-arrested S. cerevisiae at single-nucleosome resolution over 4% of the genome, and over the entire genome at lower (~265 bp) resolution. We find that nucleosomes at promoters are replaced more rapidly than at coding regions, and that replacement rates over coding regions correlate with polymerase density. In addition, rapid histone turnover is found at known chromatin boundary elements. These results suggest that rapid histone turnover serves to functionally separate chromatin domains and prevent spread of histone states. Keywords: Chip-chip, PolII

2007-05-16 | GSE6667 | GEO

p65

Project description:p65

| PRJNA811604 | ENA

PolII occupancy

2007-05-16 | E-GEOD-6667 | biostudies-arrayexpress

BRD4 Prevents the Accumulation of R-loops and Protects Against Transcription-Replication Collision Events and DNA Damage

Project description:Brd4 is essential in enhancing productive polII elongation along gene bodies. Inhibition of Brd4 leads to transcription-replciation conflicts and DNA damage.

2020-06-15 | GSE151038 | GEO

Expression data from macrophages from control and BRD4 conditional KO mice

Project description:Expression data from macrophages from control and BRD4 conditional KO mice

| PRJNA413389 | ENA

ChIP-seq analysis of BRD4, MED1 and P65 in SCC1 cells after JQ1 treatment

Project description:ChIP-seq analysis of BRD4, MED1 and P65 in SCC1 cells after JQ1 treatment

| PRJNA544484 | ENA

POLII localization in HeLa cells

Project description:RNA polymerase II (POLR2A; POLII) was localized via ChIP-chip using N20 Ab-mediated pull-down of POLII Keywords: ChIP-chip

2006-03-09 | GSE4337 | GEO

Expression data from activated peritoneal macrophages from control and BRD4 conditional KO mice

Project description:Expression data from activated peritoneal macrophages from control and BRD4 conditional KO mice