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Gossypium

ABSTRACT: Cotton genome sequencing project

PROVIDER: PRJNA450479 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR7887410_1.fastq.gz	Fastqsanger.gz
	SRR7887410_2.fastq.gz	Fastqsanger.gz
	SRR7887420_1.fastq.gz	Fastqsanger.gz
	SRR7887420_2.fastq.gz	Fastqsanger.gz
	SRR7887411_1.fastq.gz	Fastqsanger.gz

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Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress in leaf tissue.

Project description:Transcriptome analysis in cotton under drought stress. To study the molecular response of drought stress in cotton under field condition global gene expression analysis was carried out in leaf tissue. Gossypium hirsutum cv. Bikaneri Nerma was used for the gene expression analysis. Cotton plants were subjected to drought stress at peak flowering stage. Leaf samples were collected when the soil moisture content was 19.5% which is 50% of the normal control plots. Gene expression profiles in drought induced and their respective control samples were analyzed using Affymertix cotton Genechip Genome arrays to study the global changes in the expression of genome.

2012-06-04 | GSE29566 | GEO

Affymetrix Cotton Genome array expression data of cotton fiber at different developmental stages from different varieties of Gossypium hirsutum

Project description:Cotton fiber were used for the expression analysis at different developmental stages Affymetrix Cotton Genome array were used for the global profiling of gene expression of cotton fiber at different developmental stages

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Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress during fibre development stages.

Project description:Transcriptome analysis in cotton during fibre development stages. To study the molecular response of drought stress in cotton under field condition global gene expression analysis was carried out at fibre development stages (0, 5, 10 and 20 dpa/Days post anthesis). Gossypium hirsutum cv. Bikaneri Nerma was used for the gene expression analysis. Cotton plants were subjected to drought stress at peak flowering stage. Samples were collected when the soil moisture content was 19.5% which is 50% of the normal control plots. Gene expression profiles in drought induced and their respective control samples were analyzed using Affymertix cotton Genechip Genome arrays to study the global changes in the expression of genome.

2012-06-04 | GSE29567 | GEO

High-resolution survey of transcriptome from Wild type Croker 312 and Phytochrome-A1D (PhyA1D) RNAi cotton (Gossypium hirsutum) lines

Project description:Cotton (Gossypium hirsutum) is the major contributor of feedstock for the fabric industry and thus building genomic resources in cotton such as this study are a way to understand the cotton plant's biology. Cotton cultivars that suppress PHYA1D (PhyA1 homeolog on the D genome of a tetraploid) exhibit early-flowering, increased fiber length and increased seed yield. In our proposed study, flower buds (also called squares) samples were collected from control (Croker 312 wildtype line) and RNAi lines carrying the PhyA1D suppression. RNA samples from the two lines including three biological replicates were subjected to RNA-seq sequencing to elucidate the transcriptome profile.

2022-12-02 | E-MTAB-5848 | biostudies-arrayexpress

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Project description:Tetraploid Cotton Genome sequencing and assembly

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Identification of anther expressing genes in cotton (Gossypium hirsutum)

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Identification of anther expressing genes in cotton (Gossypium hirsutum)

Project description:This study was initiated with the objective of identifying the anther/tapetum specific promoters from cotton floral buds. Cotton is an important commercial crop. Hybrid cotton varieties are developed to obtain improved yield and fiber quality. Most of the hybrid seed production in cotton is carried out by hand emasculation, which requires large amount of manpower, resulting in high cost of hybrid seed. We are developing barnase-barstar based male sterility system, which would be a better alternative for hybrid development. The tapetum specific promoters are main requirement for such a system. The study was thus carried out to identify genes expressed in the anthers. Cotton bud sizes were correlated with tapetum development. RNA was isolated from following tissues: • Anther tissues from buds at pre-meiotic stage of development (Tapetum absent) • Buds without anther tissues at pre-meiotic stage of development • Anther tissues from buds during meiosis (Tapetum present) • Buds without anther tissues during meiosis • Anther tissues from buds at post-meiotic stage of development (Tapetum degenerated) • Buds without anther tissues at post-meiotic stage of development • Leaf tissues • Seedling 5 days after germination Biotin labeled cRNA was hybridized on Affymertix cotton Genechip Genome array following Affymetrix protocols. Three biological replicates were maintained.

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Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress in leaf tissue.

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