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Structural biochemistry of the 180 kDa Arp8-module of INO80 defines its function during chromatin remodelling

ABSTRACT: Structural biochemistry of the 180 kDa Arp8-module of INO80 defines its function during chromatin remodelling

PROVIDER: PRJNA451025 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7042010.fastq.gz	Fastqsanger.gz
	SRR7042011.fastq.gz	Fastqsanger.gz
	SRR7042002.fastq.gz	Fastqsanger.gz
	SRR7042003.fastq.gz	Fastqsanger.gz
	SRR7042004.fastq.gz	Fastqsanger.gz

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Structural biochemistry of the 180 kDa Arp8-module of INO80 defines its function during chromatin remodelling

Project description:Nuclear actin (N-actin) and actin-related proteins (Arps) are critical components of several chromatin modulating complexes, including the chromatin remodeler INO80, but their function is largely elusive. Here, we report the crystal structure of the 180 kDa Arp8-module of S. cerevisiae INO80 and establish its role as an extranucleosomal DNA binding element. The actin-fold of Arp8 engages N-actin in a novel “side-to-front” interaction and specifies thereby recruitment of the Arp4-N-actin heterodimer to the segmented, “two plug” scaffold of the helical HSA domain of Ino80. The HSA domain spans a distance over 120 Å and provides a binding platform for extranucleosomal DNA, which is required for nucleosome sliding and genome-wide nucleosome positioning. Together with the recent cryoEM structure of INO80Core-nucleosome complex, our results provide insights into the mechanism by which INO80 senses 40 bp extranucleosomal entry DNA to conduct highly processive chromatin remodelling.

2018-08-14 | GSE113401 | GEO

INO80 Chromatin Remodelling Coordinates Metabolic Homeostasis with Cell Division

Project description:We performed RNA-seq across the yeast metabolic cycle (YMC) in wild-type and a strain lacking ARP5, a central component of the INO80 chromatin remodelling complex. We also perform ATAC-seq on the same samples to examine chromatin architecture across the YMC.

2018-01-02 | GSE101290 | GEO

Genetic dissection of chromatin regulators reveals the INO80 chromatin remodeling complex as a key metabolic regulator utilizing TORC1 and histone acetylation pathways

Project description:We performed RNA-seq in wild-type yeast, a strain lacking INO80, and a strain lacking ARP5, central components of the INO80 chromatin remodelling complex, treated with rapamycin

2018-02-02 | GSE103468 | GEO

Transcription profiling of IMR32 cells expressing 69-kDa or 82-kDa CHAT proteins

Project description:The goal of this study was to look at genes that were affected by 69-kDa and/or 82-kDa ChAT proteins in IMR32 cells Experiment Overall Design: The gene expression changes of IMR32 cells stably expressing either 69-kDa or 82-kDa ChAT proteins were anaylzed and compared to control IMR32 wild type cells. 3 biological replicates were anaylzed per condition (69-kDa ChAT expressing cells, 82-kDa ChAT expressing cells, or wild type IMR32 cells) for a total of 9 samples altogether.

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The INO80 complex removes H2A.Z to promote presynaptic filament formation during homologous recombination

Project description:INO80 is an evolutionary conserved nucleosome remodelling complex that acts in transcription, replication and genome stability. It is required for resistance against genotoxic agents and involved in the repair of DNA double-strand breaks (DSB) by homologous recombination (HR). However, causes underlying the HR defect of INO80-C mutant cells have been controversially discussed. We here unite previous findings using a high-resolution system to study HR in the yeast Saccharomyces cerevisiae. We find that INO80-C has at least two distinct functions during HR, which promote DNA end resection and presynaptic filament formation, respectively. Importantly, specifically the second function is linked to the histone variant H2A.Z. In the absence of H2A.Z, presynaptic filament formation and HR are restored in INO80-C-deficient mutants, suggesting that presynaptic filament formation is the crucial INO80-C function during HR.

2017-05-14 | GSE95801 | GEO

Differential gene expression of IMR32 cells expressing 69-kDa or 82-kDa CHAT proteins

Project description:The goal of this study was to look at genes that were affected by 69-kDa and/or 82-kDa ChAT proteins in IMR32 cells Keywords: comparative

2007-02-01 | GSE3506 | GEO

Knockdown of Ino80 complex subunits Actr5, Ies6, and Ino80 in RD cells

Project description:INO80 complex is an ATPase-dependent chormatin remodeling complex, which regulates various DNA metabolic processes such as DNA replication and repair. Additionally, INO80 complex also contributes to the regulation of gene expression in sterss response and development. In order to investigate the function of INO80 complex in rhabdomyosarcoma, we examined the knockdown of subunits of INO80 complex Actr5, Ies6, and Ino80 in human rhabdomyosarcoma RD cells. As a result, it was found that INO80 complex is involved in the sarcomagenicity and the disregulation of myogenic properties of rhabdomyosarcoma cells.

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