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Monitoring the mating-type-driven transcriptomes of Aspergillus fumigatus

ABSTRACT: Monitoring the mating-type-driven transcriptomes of Aspergillus fumigatus

PROVIDER: PRJNA451030 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7042050.fastq.gz	Fastqsanger.gz
	SRR7042051.fastq.gz	Fastqsanger.gz
	SRR7042052.fastq.gz	Fastqsanger.gz
	SRR7042053.fastq.gz	Fastqsanger.gz
	SRR7042054.fastq.gz	Fastqsanger.gz

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Monitoring the mating-type-driven transcriptomes of Aspergillus fumigatus

Project description:Sexual identity of the human pathogenic mold Aspergillus fumigatus is determined by the mating-type idiomorphs MAT1-1 and MAT1-2 residing at the MAT locus. Given that the MAT1 gene products are DNA binding master regulators that govern fruiting body formation, the MAT1-driven transcriptomes of A. fumigatus were monitored by conditional overexpression of either MAT1 gene followed by RNA-seq analyses.

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Transcriptomes of Aspergillus fumigatus Z5 induced by different carbon sources

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2015-11-22 | E-GEOD-55086 | biostudies-arrayexpress

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Project description:Various saprotrophic microorganisms, especially filamentous fungi, can efficiently degrade lignocellulose that is one of the most abundant natural material on earth. It consists of complex carbohydrates and aromatic polymers found in plant cell wall and thus in plant debris. Aspergillus fumigatus Z5 was isolated from compost heaps and showed highly efficient plant biomass-degradation capability.Genome analysis revealed an impressive array of genes encoding cellulases, hemicellulases, and pectinases involved in lignocellulosic biomass degradation. We sequenced the transcriptomes of Aspergillus fumigatus Z5 induced by sucrose, xylan, cellulose and rice straw, respectively. There were 444, 1711 and 1386 significantly differently (q-value ≤ 0.0001 and |log2 of the ratio of the RPM values| ≥ 2) expressed genes in xylan, cellulose and rice straw,respectively, relative to sucrose control.

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Project description:Purpose: The goal of this study is to investigate the responses of HUVECs after the stimulation of conidia of A. fumigatus Methods: HUVECs were stimulated with conidia of Aspergillus fumigatus for 2 and 6 hours. Three biological repeats of stimulated cells or un-stimulated controls were send for RNA sequencing. Results: Using an optimized data analysis workflow, we mapped about 40 million sequence reads per sample to the human genome (build hg38) and identified round 80,000 transcripts in the HUVECs upon stimulation. Conclusions: Our resutls showed the detailed analysis of HUVECs transcriptomes upton conidia of Aspergillus fumigatus stimulation.

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mating response genes

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