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Transcriptional profiling of Zygosaccharomyces bailii early response to acetic acid or copper stresses mediated by ZbHaa1

ABSTRACT: Transcriptional profiling of Zygosaccharomyces bailii early response to acetic acid or copper stresses mediated by ZbHaa1

PROVIDER: PRJNA451495 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7058070.fastq.gz	Fastqsanger.gz
	SRR7058080.fastq.gz	Fastqsanger.gz
	SRR7058071.fastq.gz	Fastqsanger.gz
	SRR7058081.fastq.gz	Fastqsanger.gz
	SRR7058072.fastq.gz	Fastqsanger.gz

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Transcriptional profiling of Zygosaccharomyces bailii early response to acetic acid or copper stresses mediated by ZbHaa1

Project description:The non-conventional yeast species Zygosaccharomyces bailii is remarkably tolerant to acetic acid, a highly important microbial inhibitory compound in Food Industry and Biotechnology. ZbHaa1 is the functional homologue of S. cerevisiae Haa1 and a bifunctional transcription factor able to modulate Z. bailii adaptive response to both acetic acid and copper stresses. RNA-Seq was used to investigate genomic transcription changes in Z. bailii during early response to sublethal concentrations of acetic acid (140 mM, pH 4.0) or copper (0.08 mM), and uncover the regulatory network activated by these stresses under ZbHaa1 control.

2018-12-21 | GSE113552 | GEO

Zygosaccharomyces bailii

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Zygosaccharomyces bailii IST302

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Zygosaccharomyces bailii strain:CYU23

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Zygosaccharomyces bailii

Project description:Transcriptome sequencings of Zygosaccharomyces bailii for its function and metabolism in single and mixed cultures

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Zygosaccharomyces bailii ISA1307 genome

Project description:The genome sequence of the spoilage yeast Zygosaccharomyces bailii ISA1307

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Fungi strain:Zygosaccharomyces bailii | isolate:Kyoho grape | breed:Zygosaccharomyces | cultivar:Microbiology

Project description:Fungi strain:Zygosaccharomyces bailii | isolate:Kyoho grape | breed:Zygosaccharomyces | cultivar:Microbiology Raw sequence reads

| PRJNA776099 | ENA

Sphingolipids contribute to acetic acid resistance in Zygosaccharomyces bailii.

Project description:Lignocellulosic raw material plays a crucial role in the development of sustainable processes for the production of fuels and chemicals. Weak acids such as acetic acid and formic acid are troublesome inhibitors restricting efficient microbial conversion of the biomass to desired products. To improve our understanding of weak acid inhibition and to identify engineering strategies to reduce acetic acid toxicity, the highly acetic-acid-tolerant yeast Zygosaccharomyces bailii was studied. The impact of acetic acid membrane permeability on acetic acid tolerance in Z. bailii was investigated with particular focus on how the previously demonstrated high sphingolipid content in the plasma membrane influences acetic acid tolerance and membrane permeability. Through molecular dynamics simulations, we concluded that membranes with a high content of sphingolipids are thicker and more dense, increasing the free energy barrier for the permeation of acetic acid through the membrane. Z. bailii cultured with the drug myriocin, known to decrease cellular sphingo-lipid levels, exhibited significant growth inhibition in the presence of acetic acid, while growth in medium without acetic acid was unaffected by the myriocin addition. Furthermore, following an acetic acid pulse, the intracellular pH decreased more in myriocin-treated cells than in control cells. This indicates a higher inflow rate of acetic acid and confirms that the reduction in growth of cells cultured with myriocin in the medium with acetic acid was due to an increase in membrane permeability, thereby demonstrating the importance of a high fraction of sphingolipids in the membrane of Z. bailii to facilitate acetic acid resistance; a property potentially transferable to desired production organisms suffering from weak acid stress.

| S-EPMC5064642 | biostudies-literature

The Zygosaccharomyces bailii transcription factor Haa1 is required for acetic acid and copper stress responses suggesting subfunctionalization of the ancestral bifunctional protein Haa1/Cup2.

Project description:BackgroundThe food spoilage yeast species Zygosaccharomyces bailii exhibits an extraordinary capacity to tolerate weak acids, in particular acetic acid. In Saccharomyces cerevisiae, the transcription factor Haa1 (ScHaa1) is considered the main player in genomic expression reprogramming in response to acetic acid stress, but the role of its homologue in Z. bailii (ZbHaa1) is unknown.ResultsIn this study it is demonstrated that ZbHaa1 is a ScHaa1 functional homologue by rescuing the acetic acid susceptibility phenotype of S. cerevisiae haa1Δ. The disruption of ZbHAA1 in Z. bailii IST302 and the expression of an extra ZbHAA1 copy confirmed ZbHAA1 as a determinant of acetic acid tolerance. ZbHaa1 was found to be required for acetic acid stress-induced transcriptional activation of Z. bailii genes homologous to ScHaa1-target genes. An evolutionary analysis of the Haa1 homologues identified in 28 Saccharomycetaceae species genome sequences, including Z bailii, was carried out using phylogenetic and gene neighbourhood approaches. Consistent with previous studies, this analysis revealed a group containing pre-whole genome duplication species Haa1/Cup2 single orthologues, including ZbHaa1, and two groups containing either Haa1 or Cup2 orthologues from post-whole genome duplication species. S. cerevisiae Cup2 (alias Ace1) is a transcription factor involved in response and tolerance to copper stress. Taken together, these observations led us to hypothesize and demonstrate that ZbHaa1 is also involved in copper-induced transcriptional regulation and copper tolerance.ConclusionsThe transcription factor ZbHaa1 is required for adaptive response and tolerance to both acetic acid and copper stresses. The subfunctionalization of the single ancestral Haa1/Cup2 orthologue that originated Haa1 and Cup2 paralogues after whole genome duplication is proposed.

| S-EPMC5234253 | biostudies-literature

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