Project description:HCMV latnecy within hematopoetic precursor cells was evaluated by infecting cells and establishing latency using a recombinant virus that allows for labeing of RNA species only in the context of a viral infection thereby allowing purification of these transcripts from mock infected cells. We used microarrays to detail the global programme of gene expression to identify changes in host transcription during HCMV latency.
Project description:Purpose: to compare host gene expression in HCMV infected and mock infected Kasumi-3 cells at 24 hours post infection and analyze viral chromatin accessibility
Project description:Purpose: to compare Pol2 occupancy of the host genome in HCMV infected and mock infected Kasumi-3 cells at 24 hours post infection and analyze Pol2 occupancy of the viral genome in GFP+ infected cells
Project description:Purpose: to compare H3K27Ac and H3K27me3 in HCMV infected and mock infected Kasumi-3 cells at 24 hours post infection and analyze H3K27Ac and H3K27me3 occupancy of the viral genome in GFP+ infected cells