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Extensive Transcriptional and Translational Regulation Occur During the Maturation of Malaria Parasite Sporozoites

ABSTRACT: Extensive Transcriptional and Translational Regulation Occur During the Maturation of Malaria Parasite Sporozoites

PROVIDER: PRJNA453153 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7059090.fastq.gz	Fastqsanger.gz
	SRR7059100.fastq.gz	Fastqsanger.gz
	SRR7059091.fastq.gz	Fastqsanger.gz
	SRR7059101.fastq.gz	Fastqsanger.gz
	SRR7059092.fastq.gz	Fastqsanger.gz

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Extensive Transcriptional and Translational Regulation Occur During the Maturation of Malaria Parasite Sporozoites

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Project description:Malaria is a mosquito-borne infectious disease of humans and other animals. It is caused by the parasitic protozoan, Plasmodium. Sporozoites, the infectious form of malaria parasites, are quiescent when they remain in the salivary glands of the Anopheles mosquito until transmission into a mammalian host. Metamorphosis of the dormant sporozoite to its active form in the liver stage requires transcriptional and translational regulations. Here, we summarize recent advances in the translational repression of gene expression in the malaria sporozoite. In sporozoites, many mRNAs that are required for liver stage development are translationally repressed. Phosphorylation of eukaryotic Initiation Factor 2α (eIF2α) leads to a global translational repression in sporozoites. The eIF2α kinase, known as Upregulated in Infectious Sporozoite 1 (UIS1), is dominant in the sporozoite. The eIF2α phosphatase, UIS2, is translationally repressed by the Pumilio protein Puf2. This translational repression is alleviated when sporozoites are delivered into the mammalian host.

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Project description:Plasmodium sporozoites are transmitted from infected mosquitoes to mammals, and must navigate the host skin and vasculature to infect the liver. This journey requires distinct proteomes. Here, we report the dynamic transcriptomes and proteomes of both oocyst sporozoites and salivary gland sporozoites in both rodent-infectious Plasmodium yoelii parasites and human-infectious Plasmodium falciparum parasites. The data robustly define mRNAs and proteins that are upregulated in oocyst sporozoites (UOS) or upregulated in infectious sporozoites (UIS) within the salivary glands, including many that are essential for sporozoite functions in the vector and host. Moreover, we find that malaria parasites use two overlapping, extensive, and independent programs of translational repression across sporozoite maturation to temporally regulate protein expression. Together with gene-specific validation experiments, these data indicate that two waves of translational repression are implemented and relieved at different times during sporozoite maturation, migration and infection, thus promoting their successful development and vector-to-host transition.

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Addendum: Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites.

Project description: Not available

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Project description:Transcriptiome of ΔPfDNMT parasite during malaria parasite asexual development [DNMT_RNAseq]

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