Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ribosome profiling Seq of mouse adult cortex after acute stress

ABSTRACT: Ribosome profiling Seq of mouse adult cortex after acute stress

PROVIDER: PRJNA453992 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7074410.fastq.gz	Fastqsanger.gz
	SRR7074420.fastq.gz	Fastqsanger.gz
	SRR7074430.fastq.gz	Fastqsanger.gz
	SRR7074411.fastq.gz	Fastqsanger.gz
	SRR7074421.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Ribosome profiling Seq of mouse adult cortex after acute stress

Project description:N6-Methyladenosine (m6A) and N6,2′-O-dimethyladenosine (m6Am) are abundant mRNA modifications that regulate transcript processing and translation. The role of both, here termed m6A/m, in the stress response in the adult brain in vivo are currently unknown. Here, we provide ribosome profiling data of 10-12 w male C57BL/6 mouse cortex of unstressed or acutely stressed mice (4 h after 15 min restraint stress) of 6 samples per condition to relate to m6A/m-Seq data of similiar samples.

2018-07-10 | GSE113789 | GEO

m6A/m-Seq of mouse adult cortex after acute stress

Project description:m6A/m-Seq of mouse adult cortex after acute stress

| PRJNA453975 | ENA

m6A/m-Seq of mouse adult cortex after acute stress

Project description:N6-Methyladenosine (m6A) and N6,2′-O-dimethyladenosine (m6Am) are abundant mRNA modifications that regulate transcript processing and translation. The role of both, here termed m6A/m, in the stress response in the adult brain in vivo are currently unknown. Here, we provide a detailed analysis of the stress-epitranscriptome using m6A/m-Seq of 10-12 w male C57BL/6 mouse cortex of unstressed or acutely stressed mice (4 h after 15 min restraint stress) of 6-7 samples per condition (pooled from 3 mice each). PolyA-RNA-fragments of each sample was processed both as m6A/m-sample (RNA immunoprecipiation RIP with an m6A and m6Am antibody) and RNA-input sample.

2018-07-10 | GSE113781 | GEO

Acrylamide acute neurotoxicity in adult zebrafish

Project description:Acute exposure to acrylamide (ACR), a type-2 alkene, may lead to a ataxia, skeletal muscles weakness and numbness of the extremities in exposed human and laboratory animals. Recently, a zebrafish model for ACR neurotoxicity mimicking most of the pathophysiological processes described in mammalian models, was generated in 8 days post-fertilization larvae. In order to better understand the predictive value of the zebrafish larvae model of acute ACR neurotoxicity, in the present manuscript the ACR acute neurotoxicity has been characterized in the brain of adult zebrafish, and the results compared with those obtained with the whole-larvae. Although qualitative and quantitative analysis of the data shows important differences in the ACR effects between the adult brain and the whole-larvae, the overall effects of ACR in adult zebrafish, including a significant decrease in locomotor activity, altered expression of transcriptional markers of proteins involved in synaptic vesicle cycle, presence of ACR-adducts on cysteine residues of some synaptic proteins, and changes in the profile of some neurotransmitter systems, are similar to those described in the larvae. Thus, these results support the suitability of the zebrafish ACR acute neurotoxicity recently developed in larvae for screening of molecules with therapeutic value to treat this toxic neuropathy.

2018-10-19 | PXD008993 | Pride

Transcriptome-wide profiling of acute stress induced changes in ribosome occupancy level using external standards

Project description:Transcriptome-wide profiling of acute stress induced changes in ribosome occupancy level using external standards

| PRJNA876935 | ENA

Chronic social instability stress during adolescence does not change the acute stress response in adult male mice

Project description:Chronic social instability stress during adolescence does not change the acute stress response in adult male mice

| PRJNA723330 | ENA

Pde4b-regulated cAMP signaling pathway in the AUDGABA-S1TrSst circuit underlies acute stress-induced anxiety-like behavior

Project description:Acute stress-induced anxiety is an important way for animals to avoid danger. However, neural and molecular mechanisms that underlie control of anxiety behavior are largely elusive. Here, we find acute physical stress activates a large number of neurons in the primary somatosensory cortex, trunk region (S1Tr). Single-cell sequencing reveals the S1Tr c-fos positive neurons activated by acute stress are largely GABAergic somatostatin (Sst) neurons. These S1TrSst neurons activated by acute stress showed desensitization during subsequent anxiety-like behavior tests. Selective inhibition or apoptosis of S1TrSst neurons mimics acute stress effects to induce anxiety. In contrast, selective activation of S1TrSst neurons reduced acute stress-induced anxiety. Furthermore, we demonstrate that S1TrSst cells receive inputs from the secondary auditory cortex, dorsal area (AUD) GABAergic neurons to modulate acute stress-induced anxiety. Finally, from the results of spatial transcriptome sequencing and precise projection-specificity Pde4b protein knockdown strategy, we show that acute stress reduces Pde4b-regulated cyclic adenosine monophosphate (cAMP) signaling pathway activity in the AUDGABA-S1TrSst projections and resulting in a hypoactivity of S1TrSst neurons during subsequent behavioral tests. Our study unveils a neural and molecular mechanism for acute stress-elicited anxiety and affords a theoretical basis for clinical treatment of anxiety disorders.

2025-01-02 | GSE285495 | GEO