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Gossypium hirsutum

ABSTRACT: Upland cotton Transcriptome

PROVIDER: PRJNA454052 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7081570_1.fastq.gz	Fastqsanger.gz
	SRR7081570_2.fastq.gz	Fastqsanger.gz
	SRR7081571.fastq.gz	Fastqsanger.gz
	SRR7081572.fastq.gz	Fastqsanger.gz
	SRR7081563_1.fastq.gz	Fastqsanger.gz

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Project description:In this study, the leaves of upland cotton X142 and its corresponding fuzzness-lintness mutant X142fl were used as materials. Proteomic techniques were used to analysis the protein abundant between the leaves of upland cotton and MaxQuant software was selected to analyze the proteomic data.

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Transcriptome profiling of Gossypium hirsutum in response to NO by high-throughput tag-sequencing

Project description:Nitric oxide(NO) is emerging as an important signaling molecule with diverse physiological functions in plants, leading to a necessity to discover the downstream genes and signal transduction network modulated by NO. In the current study, changes in gene expression in response to NO were studied in upland cotton(Gossypium hirsutum) using Solexa/Illumina’s digital gene expression (DGE) system, a tag-based transcriptome sequencing method. After mapping to an integrated reference sequence database, we obtained about 30,000 genes per library, and yielded 2,393 differential expressed genes in total.

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cDNA expression profiles of +7 DPA wild-type upland cotton (Gossypium hirsutum cv. Xuzhou142) ovules over the one-year cycle

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Identification of candidate thermotolerance genes during early seedling stage in upland cotton (Gossypium hirsutum L.) revealed by comparative transcriptome analysis

Project description:High temperature (HT) stress is a major environmental stress that limits cotton growth, metabolism, and yield worldwide. The identification and characterization of thermotolerance is restricted by the plant growth environment and growth stage. In this study, four genotypes of upland cotton (Gossypium hirsutum L.) with known field thermotolerance were evaluated under normal and HTs at the seedlings stage in a growth cabinet with 11 physiological, biochemical, and phenotypic assays. Consistent with previous field observations, the thermotolerance could be identified by genotype differences at the seedling stage under HT in a growth cabinet. Comparative transcriptome analysis was performed on seedlings of two contrasting cotton genotypes after 4 and 8 hours of HT exposure. Gene ontology analysis combined with BLAST annotations revealed a large number of HT-induced differentially expressed genes (4,698) that either exhibited higher expression levels in the heat-tolerant genotype (Nan Dan Ba Di Da Hua) compared with the heat-sensitive genotype (Earlistaple 7), or were differentially expressed only in Nan Dan Ba Di Da Hua. These genes encoded mainly protein kinases, transcription factors, and heat shock proteins, which were considered to play key roles in thermotolerance in upland cotton. Two heat shock transcription factor genes (homologs of AtHsfA3, AtHsfC1) and AP2/EREBP family genes (homologs of AtERF20, AtERF026, AtERF053, and AtERF113) were identified as possible key regulators of thermotolerance in cotton. Some of the differentially expressed genes were validated by quantitative real-time PCR analysis. Our findings provide candidate genes that could be used to improve thermotolerance in cotton cultivars.

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