Project description:Single cell RNA sequencing of sorted Cd45.2 colonic macrophage from bone marrow chimeras from IL-10R KO or IL-10R/CerS2 DKO donors

2024-01-11 | GSE252548 | GEO

Determining sequencing depth in a single-cell RNA-seq experiment.

Project description:An underlying question for virtually all single-cell RNA sequencing experiments is how to allocate the limited sequencing budget: deep sequencing of a few cells or shallow sequencing of many cells? Here we present a mathematical framework which reveals that, for estimating many important gene properties, the optimal allocation is to sequence at a depth of around one read per cell per gene. Interestingly, the corresponding optimal estimator is not the widely-used plug-in estimator, but one developed via empirical Bayes.

| S-EPMC7005864 | biostudies-literature

single-cell RNA-seq experiment to study mouse stomach metaplastic organoids

Project description:single-cell RNA-seq experiment to study mouse stomach metaplastic organoids

| PRJNA1123833 | ENA

Single-cell RNA-sequencing of mouse colonic monocytes and macrophages

Project description:Single-cell RNA-sequencing of mouse colonic monocytes and macrophages

| PRJNA543535 | ENA