Project description:The goal of this study was to identify changes in the gene expression profile (GEP) of HeLa cells in response to induction of expression of DOCK10 during 24 hours.
Project description:In order to identify the effects of TFEB overexpression on the hela cells transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the hela TFEB stable clones Transcriptome analysis of hela stable clones overexpressing TFEB-GFP
Project description:In order to identify the effects of TFEB overexpression on the hela cells transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the hela TFEB stable clones Transcriptome analysis of hela stable clones overexpressing TFEB-3xFLAG
Project description:In order to identify novel genes regulated by p53, stable line containing tet-on inducible p53 construct was generated and used for gene expression analysis. Tet-on p53 inducible stable line was induced by doxycyclin for 24 hours before total RNA was extracted for array analysis.
Project description:Stable inducible neurons were generated using Tol2 recombinase to generate human embryonic stem cells that expressed human NEUROG2 in a doxycycline regulated maner. Treatment with doxycycline caused neuronal differentiation of the stem cells (samples 55649-55653). The post-mitotic excitatory neurons derived from the stem cells were used to examine FGF2 regulation of gene expression (samples 57089-57096).
Project description:In order to identify novel genes regulated by p53, stable line containing tet-on inducible p53 construct was generated and used for gene expression analysis.
Project description:The host differential expression protein profile caused by the stable transfection of the CT143 gene into HeLa cells was successfully constructed. These differential proteins may interact with each other and participate in the regulation of intracellular signal transduction, cell secretion, protein processing and hydrolysis, enzyme activity, and other important biological processes, suggesting that the CT143 protein may affect the biological behavior of the host cell by altering the expression of host proteins, thus interfering with the growth and development of Ct.