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Growth enhancement of obligate anaerobic archaeon, Thermococcus onnurineus NA1 by the addition of oxygen

ABSTRACT: Growth enhancement of obligate anaerobic archaeon, Thermococcus onnurineus NA1 by the addition of oxygen

PROVIDER: PRJNA470757 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7142300.fastq.gz	Fastqsanger.gz
	SRR7142299.fastq.gz	Fastqsanger.gz

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Similar Datasets

Growth enhancement of obligate anaerobic archaeon, Thermococcus onnurineus NA1 by the addition of oxygen

Project description:Hyperthermophilic archeaon, Thermococcus onnurineus NA1 has known as a strict anaerobe. To date, a few of studies have been reported that strict anaerobe can grow using oxygen. However, the research of the growth enhancement of strict anaerobic archaeon belonging to the order of Thermococcales using the oxygen, in which has never been reported so far. In this study, we showed that the growth of T. onnurineus NA1 strain increased under various oxygen concentrations and we observed that oxygen was decreased in the headspace during the growth of cell. Genome-wide transcriptomic analysis was carried out to evaluate alterations in gene expression induced by O2 and to explain the physiological effects of oxidative stress on the growth of T. onnurineus NA1.

2021-03-10 | GSE114281 | GEO

Thermococcus onnurineus NA1

Project description:Expression analysis of Thermococcus onnurineus NA1 KCTC10859 and an Frh-deficient mutant, Frh overexpression mutant and TON_0282 deletion mutant in a T. onnurineus NA1 strain.

| PRJNA348351 | ENA

Expression analysis of Thermococcus onnurineus NA1 KCTC10859 and an Frh-deficient mutant, Frh overexpression mutant and TON_0282 deletion mutant in a T. onnurineus NA1 strain.

Project description:Investigation of whole genome gene expression level changes in an Frh-deficient mutant, Frh overexpression mutant and TON_0282 deletion mutant, compared to the wild-type strain. In this study, as a first step to assess the role of the frhAGB-encoding hydrogenases and TON_0282 gene, we constructed three mutants by deleting each gene in a T. onnurineus NA1.

2018-02-19 | GSE88718 | GEO

Increased biohydrogen production by deletion of uncharacterized DNA-binding protein discovered in hyperthermophilic archaeon Thermococcus onnurineus NA1

Project description:In ordet to understand in vivo role of uncharacterized DNA-binding protein (TON_1525) in T. onnurineus NA1, TON_1525 gene deletion mutant was constructed. Also transcriptome analysis was perfomed between wild type and mutant strains to measure changed expression profile of genes by deletion of TON_1525 gene under CO supplemented medium.

2023-09-11 | GSE164395 | GEO

Thermococcus onnurineus

Project description:Thermococcus onnurineus overview

| PRJNA36273 | ENA

Thermococcus onnurineus NA1

Project description:Screening of a novel strong promoter by RNA sequencing and its application to H2 production in a hyperthermophilic archaeon

| PRJNA269922 | ENA

Thermococcus onnurineus NA1

Project description:Hyperthermophilic bacterium

| PRJNA20773 | ENA

Thermococcus onnurineus NA1

Project description:Experimental evolution of a hyperthermophilic archaeon on carbon monoxide enhanced hydrogen productivity through novel mechanisms associated with genome, transcriptome and epigenome changes

| PRJNA296014 | ENA

Biohydrogen production of obligate anaerobic archaeon Thermococcus onnurineus NA1 under oxic conditions via overexpression of frhAGB-encoding hydrogenase genes.

Project description:Background:The production of biohydrogen (H2) as a promising future fuel in anaerobic hyperthermophiles has attracted great attention because H2 formation is more thermodynamically feasible at elevated temperatures and fewer undesired side products are produced. However, these microbes require anoxic culture conditions for growth and H2 production, thereby necessitating costly and time-consuming physical or chemical methods to remove molecular oxygen (O2). Therefore, the development of an O2-tolerant strain would be useful for industrial applications. Results:In this study, we found that the overexpression of frhAGB-encoding hydrogenase genes in Thermococcus onnurineus NA1, an obligate anaerobic archaeon and robust H2 producer, enhanced O2 tolerance. When the recombinant FO strain was exposed to levels of O2 up to 20% in the headspace of a sealed bottle, it showed significant growth. Whole transcriptome analysis of the FO strain revealed that several genes involved in the stress response such as chaperonin ? subunit, universal stress protein, peroxiredoxin, and alkyl hydroperoxide reductase subunit C, were significantly up-regulated. The O2 tolerance of the FO strain enabled it to grow on formate and produce H2 under oxic conditions, where prior O2-removing steps were omitted, such as the addition of reducing agent Na2S, autoclaving, and inert gas purging. Conclusions:Via the overexpression of frhAGB genes, the obligate anaerobic archaeon T. onnurineus NA1 gained the ability to overcome the inhibitory effect of O2. This O2-tolerant property of the strain may provide another advantage to this hyperthermophilic archaeon as a platform for biofuel H2 production.

| S-EPMC6367845 | biostudies-literature

Crystallization and preliminary X-ray crystallographic analysis of Lon from Thermococcus onnurineus NA1.

Project description:Lon is an oligomeric ATP-dependent protease that degrades defective or denatured proteins as well as some folded proteins for the control of cellular protein quality and metabolism. Lon from Thermococcus onnurineus NA1 was purified and crystallized at 295 K. A 2.0 A resolution data set was collected using synchrotron radiation. The crystals belonged to space group P6(3), with unit-cell parameters a = 121.45, b = 121.45, c = 195.24 A. Assuming the presence of two monomers in the asymmetric unit, the solvent content was estimated to be about 60.7%.

| S-EPMC2805537 | biostudies-literature

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