Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Novel human full-thickness, three-dimensional non-keratinized mucous membrane model for pharmacological studies in wound healing

ABSTRACT: Novel human full-thickness, three-dimensional non-keratinized mucous membrane model for pharmacological studies in wound healing

PROVIDER: PRJNA471242 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Novel human full-thickness, three-dimensional non-keratinized mucous membrane model for pharmacological studies in wound healing

Project description:Efforts are increasingly aiming to develop in vitro models that can provide effective alternatives to in vivo experiments. The main aim of this study was the establishment of an in vitro model of the non-keratinized mucous membrane that can be used as a standardized tool to evaluate biological and therapeutic effects of pharmaceuticals for mucosal wound healing. Performing histological and immunofluorescence analyses with known differentiation markers we proved that our model mimics the two distinctive layers of the mucous membrane – the stratified squamous epithelium and the lamina propria. In our study we used our model to investigate molecular effects of a dexpanthenol-containing ointment that is widely used in the wound treatment of the oral mucosa. For that purpose our model exhibits a unique feature in that dexpanthenol and proliferation enhancing additives that may interfere with our studies are not required for the maintenance of the model culture. After setting standardized lesions with a CO2 laser, topical treatment with the dexpanthenol-containing ointment enhanced wound closure in our non-keratinized mucous membrane model compared to placebo and untreated controls. Furthermore, microarray analysis revealed that the treatment of our laser wounded model with the dexpanthenol-containing ointment evoked an upregulated expression of various genes related to accelerated wound healing. Overall, we verified that our mucous membrane model can be utilized in future to monitor ex vivo effects of various topical therapies on mucosa morphology, physiology, and gene expression. Our findings confirm the potential of the non-keratinized mucous membrane model as an in vitro tool for the replacement of pharmacological in vivo studies regarding mucosal wound healing.

2018-05-15 | GSE114378 | GEO

Novel human full-thickness three-dimensional non-keratinized mucous membrane model for pharmacological studies in wound healing

2018-08-25 | GSE119035 | GEO

Novel human full-thickness three-dimensional non-keratinized mucous membrane model for pharmacological studies in wound healing

Project description:Novel human full-thickness three-dimensional non-keratinized mucous membrane model for pharmacological studies in wound healing

| PRJNA487804 | ENA

Endoscopic Full Thickness Resection in the Lower GI Tract With the "Full Thickness Resection Device"

Project description:Observational prospective multicenter study to investigate efficacy and safety of endoscopic full thickness resection in the lower GI tract using a novel over-the-scope full thickness resection device.

| 2183735 | ecrin-mdr-crc

Investigation of the molecular effects of a dexpanthenol-containing ointment and liquid in the aftercare treatment of acute radiodermatitis and mucositis using newly developed 3D models for both skin conditions

Project description:This study aimed to investigate the molecular effects of radiation and subsequent aftercare treatment with dexpanthenol-containing ointment and liquid on newly established full-thickness 3D skin models depicting acute radiodermatitis and mucositis. We established in vitro models of the non-keratinized mucous membrane as well as normal human skin models. To mimic radiomucositis and radiodermatitis, models were irradiated with 5 Gray. Afterwards, models were treated topically every second day with dexpanthenol-containing ointment or liquid in comparison to placebo and untreated controls.

2020-05-19 | GSE150738 | GEO

Endoscopic Full-Thickness Resection In Colon

Project description:Endoscopic full-thickness resection (EFTR) in the colon using an over-the-scope clip (OTSC) as a closure mechanism is a recent technique that allows the endoscopic resection of colonic lesions that are poor candidates for conventional endoscopic resection techniques. The aim is to study the safety and efficacy of EFTR in colon.

| 2294341 | ecrin-mdr-crc

transcriptional profiling of mouse full thickness skin of PPARgamma null mice

Project description:PPARgamma null (PpargΔ/Δ) mice present a generalized lipoatrophy and a dramatic skin phenotype, characterized by delayed hair morphogensis and the appearance, at adult age, of severe inflammatory infiltration. To investigate the molecular mechanisms underlying the delayed hair morphogenesis observed in PpargΔ/Δ mice, we used microarrays to detail the global program of gene expression in full thickness skin of PpargΔ/Δ mice and respective control mice at embryionic stage E17.5.

2017-07-31 | GSE85497 | GEO

Technical Failure During Colorectal Endoscopic Full Thickness Resection (EFTR): The "Through Thick and Thin" Study

Project description:Among advanced resection techniques, endoscopic full thickness resection (EFTR) allows closure and full-thickness resection by the use of the non-exposed full thickness resection device (FTRD). The study is a retrospective analysis of technical failure occurring during colorectal full-thickness resection. Full thickness resection is a safe and effective procedure for "difficult" colorectal lesions. However, technical failure can occur and to date there is no evidence about type and clinical consequences in this setting.

| 64128 | ecrin-mdr-crc

Full Thickness Resection or Endoscopic Submucosal Dissection for Difficult Colorectal Lesions.

Project description:Compare the efficacy and safety of endoscopic resection a full thickness (EFTR) with Full Thickness Resection Device (FTRD) System versus endoscopic submucosal dissection (ESD) in the treatment of Laterally Spreading Tumor Non Granular Type (LST-NG), "no lift" lesions colon and residual / relapse on scars from previous resections endoscopic colon. The dimensional cut off of these lesions is ≤30mm

| 2745298 | ecrin-mdr-crc

Deciphering the molecular effects of non-ablative Er:YAG laser treatment in an in vitro model of the non-keratinized mucous membrane

Project description:This study aimed to investigate the molecular effects of non-ablative Er:YAG laser treatment using an in vitro model of the non-keratinized mucous membrane and to compare its molecular effects with other ablative and non-ablative laser systems. In dermatology, the use of non-ablative and ablative fractional lasers has become the gold standard treatment for a number of indications. Each of the individual laser types is advantageous for different types of indications due to its respective properties, but new technologies open up new fields of application for individual laser systems. Performing a comprehensive gene expression profiling we compared the gene regulatory effects of non-ablative Er:YAG laser with other non-ablative and ablative laser systems. In vitro 3D models have proven to be a reliable and reproducible tool to study the molecular biological effects of different laser settings.

2020-06-01 | GSE151041 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data