Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Organoids derived from pancreatic adenocarcinoma patients share morphological and genetic features with the primary tumor [RNA-seq]

ABSTRACT: Organoids derived from pancreatic adenocarcinoma patients share morphological and genetic features with the primary tumor [RNA-seq]

PROVIDER: PRJNA471423 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7165140_1.fastq.gz	Fastqsanger.gz
	SRR7165140_2.fastq.gz	Fastqsanger.gz
	SRR7165150_1.fastq.gz	Fastqsanger.gz
	SRR7165150_2.fastq.gz	Fastqsanger.gz
	SRR7165160_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 84

Similar Datasets

Organoids derived from pancreatic adenocarcinoma patients share morphological and genetic features with the primary tumor

Project description:Organoids derived from pancreatic adenocarcinoma patients share morphological and genetic features with the primary tumor

| PRJNA471419 | ENA

Organoids derived from pancreatic adenocarcinoma patients share morphological and genetic features with the primary tumor [Drop-seq]

Project description:Organoids derived from pancreatic adenocarcinoma patients share morphological and genetic features with the primary tumor [Drop-seq]

| PRJNA471422 | ENA

Pancreatic adenocarcinoma human organoids share structural and genetic features with primary tumors

Project description:Pancreatic adenocarcinoma human organoids share structural and genetic features with primary tumors

| PRJNA498740 | ENA

Pancreatic adenocarcinoma human organoids share structural and genetic features with primary tumors

Project description:Pancreatic adenocarcinoma human organoids share structural and genetic features with primary tumors

| PRJNA498741 | ENA

Organoids derived from pancreatic adenocarcinoma patients share morphological and genetic features with the primary tumor [Drop-seq]

Project description:In this study, we applied Drop-seq, a high throughput single cell RNA-seq platform, to characterize the different cell types present in pancreatic cancer organoids. We sequenced the transcriptomes of 7675 single cells from organoids derived from a patient undergoing a Whipple procedure for pancreatic ductal adenocarcinoma (PDAC). We then developed a novel clustering approach based on a class of probabilistic generative models called topic models, leading to the identification of subpopulations of cells.

2020-06-01 | GSE114454 | GEO

Human Cerebral Organoids: Cellular composition and subcellular morphological features

Project description:Human Cerebral Organoids: Cellular composition and subcellular morphological features

| PRJNA1108233 | ENA

Organoids derived from pancreatic adenocarcinoma patients share morphological and genetic features with the primary tumor

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-06-01 | GSE114455 | GEO

Human Organoids Share Structural and Genetic Features with Primary Pancreatic Adenocarcinoma Tumors.

Project description:Patient-derived pancreatic ductal adenocarcinoma (PDAC) organoid systems show great promise for understanding the biological underpinnings of disease and advancing therapeutic precision medicine. Despite the increased use of organoids, the fidelity of molecular features, genetic heterogeneity, and drug response to the tumor of origin remain important unanswered questions limiting their utility. To address this gap in knowledge, primary tumor- and patient-derived xenograft (PDX)-derived organoids, and 2D cultures for in-depth genomic and histopathologic comparisons with the primary tumor were created. Histopathologic features and PDAC representative protein markers (e.g., claudin 4 and CA19-9) showed strong concordance. DNA- and RNA-sequencing (RNAseq) of single organoids revealed patient-specific genomic and transcriptomic consistency. Single-cell RNAseq demonstrated that organoids are primarily a clonal population. In drug response assays, organoids displayed patient-specific sensitivities. In addition, the in vivo PDX response to FOLFIRINOX and gemcitabine/abraxane treatments were examined, which was recapitulated in vitro with organoids. This study has demonstrated that organoids are potentially invaluable for precision medicine as well as preclinical drug treatment studies because they maintain distinct patient phenotypes and respond differently to drug combinations and dosage. IMPLICATIONS: The patient-specific molecular and histopathologic fidelity of organoids indicate that they can be used to understand the etiology of the patient's tumor and the differential response to therapies and suggests utility for predicting drug responses.

| S-EPMC6647028 | biostudies-literature

Organoids derived from pancreatic adenocarcinoma patients share morphological and genetic features with the primary tumor [RNA-seq]

Project description:RNA sequencing demonstrates molecular profiles common to primary tumor, PDX and organoid models

2020-06-01 | GSE114453 | GEO

Characterization of morphological alterations in micropapillary adenocarcinoma of the lung

Project description:Micropapillary adenocarcinoma of the lung is a cancer with poor prognosis and characterized by the presence of tumor cells with ring-like glandular structure floating within alveolar spaces. We investigated the association between its morphological, biochemical, and immunohistochemical characteristics and malignancy using the KU-Lu-MPPt3 cell line established from a patient with micropapillary adenocarcinoma. Two subpopulations of KU-Lu-MPPt3 cells, adhesive (AD) and clumpy and suspended (CS) cells, were prepared and subjected to DNA microarray.

2021-09-28 | GSE184883 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data