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Profiling of murine resident adipose tissue and aortic macrophages according to their expression status of LYVE-1

ABSTRACT: Profiling of murine resident adipose tissue and aortic macrophages according to their expression status of LYVE-1

PROVIDER: PRJNA472001 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7182300_1.fastq.gz	Fastqsanger.gz
	SRR7182300_2.fastq.gz	Fastqsanger.gz
	SRR7182301_1.fastq.gz	Fastqsanger.gz
	SRR7182301_2.fastq.gz	Fastqsanger.gz
	SRR7182292_1.fastq.gz	Fastqsanger.gz

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Profiling of murine resident adipose tissue and aortic macrophages according to their expression status of LYVE-1

Project description:Beside their role in conventional immune regulation, macrophages are now recognised as essential regulator of local tissue homeostasis depending on the tissue in which they reside. Using phenotyping, we found that LYVE-1+ macrophages are the major resident macrophage population in murine aorta and adipose tissues under steady state. Furthermore, imaging analysis revealed the exclusive association of adipose tissue LYVE-1+ macrophages with smooth muscle positive large blood vessels. Hence, we hypothesize that LYVE-1+ macrophages sustain large vessel functional homeostasis. The present experiment aims to better characterize resident LYVE-1+ vs LYVE-1- macrophages in aorta and adipose tissues.

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Transcription profile of adipose-resident macrophages and mesenchymal stem cells.

Project description:It is commonly-recognized that hematopoietic cells residing in adipose tissue (AT) exhibit a broad range of physiological functions for maintenance of homeostasis and remodeling and repair of tissues aside from the involvement in inflammation. We evaluated the cell composition of hematopoietic cells in human non-obese AT and analyzed AT-resident macrophages in detail. Liposuction aspirates were obtained from healthy donors undergoing liposuction of the abdomen and thighs. CD34-positive AT resident macrophages (ATMs), CD34-negative ATMs and adipose stromal cells were sorted from aspirated AT by a multiparameter fluorescent-activated cell sorter. Circulating monocytes were also sorted from peripheral blood.These 4 condition were analysed transcription profile.

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Low-input bulk RNA sequencing of thymic, mediastinal adipose tissue, and aortic arch CD4+CD8+ (DP) cells

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Transcription profile of adipose-resident macrophages and mesenchymal stem cells.

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