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Genome wide analysis of chick limb bud tissue treated with FGF2 for 3 hrs

ABSTRACT: Genome wide analysis of chick limb bud tissue treated with FGF2 for 3 hrs

PROVIDER: PRJNA472073 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7184313.fastq.gz	Fastqsanger.gz
	SRR7184314.fastq.gz	Fastqsanger.gz
	SRR7184315.fastq.gz	Fastqsanger.gz
	SRR7184316.fastq.gz	Fastqsanger.gz
	SRR7184317.fastq.gz	Fastqsanger.gz

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Genome wide analysis of chick limb bud tissue treated with FGF2 for 24hrs (microarray)

Project description:Genome wide analysis of chick limb bud tissue treated with FGF2 for 24hrs (microarray)

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Genome wide analysis of chick limb bud tissue treated with FGF2 for 3hrs (RNA-Seq)

Project description:Genome wide analysis of chick limb bud tissue treated with FGF2 for 3hrs (RNA-Seq)

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Genome wide analysis of chick limb bud tissue treated with FGF2 for 24hrs (microarray)

Project description:During limb development, fibroblast growth factors (FGFs) govern proximal-distal outgrowth and patterning. FGFs also synchronize developmental patterning between the proximal-distal and anterior-posterior axes by maintaining sonic hedgehog (SHH) expression in cells of the zone of polarizing activity (ZPA) in the distal posterior mesoderm. SHH, in turn, maintains FGFs in the apical ectodermal ridge (AER) which caps the distal tip of the limb bud. Crosstalk between FGF and SHH signaling is critical for patterned limb development, but the mechanisms underlying this feedback loop are not well characterized. Implantation of FGF beads in the proximal posterior limb bud can maintain SHH expression in the former ZPA domain (evident 3hrs after application), while prolonged exposure (24hrs) can induce SHH outside of this domain. Although temporally and spatially disparate, comparative analysis of transcriptome data from these different populations enriched molecules required for FGF-mediated SHH regulation.

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Genome wide analysis of chick limb bud tissue treated with FGF2 for 3hrs (RNA-Seq)

Project description:During limb development, in addition to proximal to distal outgrowth, FGF regulates a number of factors necessary for SHH expression. To elucidate the molecules involved in FGF-mediated SHH expression we implanted FGF2 beads in HH23 chicken forelimb buds and excised the SHH-expressing tissue for transcriptome analysis. The hypothesis is that this transcriptome data along with data from SHH-expressing tissue after a 24hr treatment would highlight molecules necessary for SHH FGF-mediated SHH expression. Results from the overlap provide a short-list of SHH-related genes which require further testing to assess their role in mediating SHH expression.

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microRNA-Seq of Gallus gallus embryo tissues: Undetermined Presomitic Mesoderm (PSM), Determined PSM, and Limb bud

Project description:This analysis aims at finding the microRNAs present in three chick embryo undifferentiated tissues (pools of tissues): Undetermined Presomitic Mesoderm (PSM-U), Determined Presomitic Mesoderm (PSM-D) and Distal limb bud (Limb).

2021-10-25 | E-MTAB-8176 | biostudies-arrayexpress

Genome wide analysis of chick limb bud tissue treated with FGF2 for 3 hrs

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-12-18 | GSE114663 | GEO

Bhlha9 deficiency effect in limb bud

Project description:Analysis of mouse limb bud (E10.5) lacking the Bhlha9 gene. Bhlha9 knockout mouse shows syndactyly and poliosis in the limb. This microarray results provides insight into the molecular mechanisms underlying Bhlha9 function in the limb development DNA microarray analysis was performed using Affymetrix mouse genome 430 2.0 array. RNA samples were obtained from the whole limb bud of the E10.5 wild-type and Bhlha9 knockout embryos described above. Total RNA (200 ng) was reverse-transcribed and biotinylated using the GeneChip 3â² IVT Express Kit (Affymetrix). The microarray data were summarized using the MAS 5.0 method.

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