Thymic tuft cell single-cell mRNA analysis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA472990 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR7215580.fastq.gz | Fastqsanger.gz | |||
| SRR7215590.fastq.gz | Fastqsanger.gz | |||
| SRR7215600.fastq.gz | Fastqsanger.gz | |||
| SRR7215610.fastq.gz | Fastqsanger.gz | |||
| SRR7215620.fastq.gz | Fastqsanger.gz |
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Project description:T cell development and selection is orchestrated in the thymus by a specialized niche of diverse stromal populations. By transcriptional single cell sorting, we de novo characterize the entire stromal compartment of the thymus. We identified dozens of cell states within the thymic stroma, with thymic epithelial cells (TEC) showing the highest degree of heterogeneity. Our analysis highlights four major medullary TEC (mTEC I-IV) populations, with distinct molecular functions, epigenetic landscapes and lineage regulators. Specifically, mTEC-IV constitutes a new and highly divergent TEC lineage with molecular characteristics of the gut chemosensory epithelial tuft cells. Mice deficient of Pou2f3, a tuft cells master regulator, resulted in complete and specific depletion of mTEC-IV, without affecting other TEC populations. Overall, our study comprehensively defines all stroma cells in the thymus and identifies a new TEC lineage associated with chemosensory properties that may potentially link the adaptive immune system to environmental and neurological signals.
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