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Campylobacter jejuni demonstrates conserved proteomic and transcriptomic responses when co-cultured with human INT 407 and Caco-2 epithelial cells

ABSTRACT: Campylobacter jejuni demonstrates conserved proteomic and transcriptomic responses when co-cultured with human INT 407 and Caco-2 epithelial cells

PROVIDER: PRJNA473070 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7216910_1.fastq.gz	Fastqsanger.gz
	SRR7216910_2.fastq.gz	Fastqsanger.gz
	SRR7216911.fastq.gz	Fastqsanger.gz
	SRR7216912.fastq.gz	Fastqsanger.gz
	SRR7216913_1.fastq.gz	Fastqsanger.gz

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Campylobacter jejuni demonstrates conserved proteomic and transcriptomic responses when co-cultured with human INT 407 and Caco-2 epithelial cells

Project description:Major foodborne bacterial pathogens, such as Campylobacter jejuni, have devised complex strategies to establish and foster intestinal infections. For more than two decades researchers have used immortalized cell lines derived from human intestinal tissue to dissect C. jejuni-host cell interactions. Known from these studies is that C. jejuni virulence is multifactorial, requiring a coordinated response to produce virulence factors that facilitate the bacterium’s host-cell interactions. This study was initiated to identify C. jejuni proteins that contribute to adaptation to the host cell environment and cellular invasion. We demonstrated that C. jejuni responds to INT 407 and Caco-2 cells in a similar fashion at the cellular and molecular levels. Active protein synthesis was found to be required for C. jejuni to maximally invade these host cells. Proteomic and transcriptomic approaches were then used to define the protein and gene expression profiles of C. jejuni co-cultured with cells. By focusing on those genes showing increased expression by C. jejuni when co-cultured with epithelial cells, we discovered that C. jejuni quickly adapts to co-culture with epithelial cells by synthesizing gene products that enable it to acquire specific amino acids for growth, scavenge for inorganic molecules including iron, resist reactive oxygen/nitrogen species, and promote bacteria-host cell interactions. Based on these findings, we selected a subset of the genes involved in chemotaxis and the regulation of flagellar assembly and generated C. jejuni deletion mutants for phenotypic analysis. Binding and internalization assays revealed significant differences in the interaction of C. jejuni chemotaxis and flagellar regulatory mutants. The identification of genes involved in C. jejuni adaptation to culture with host cells provides new insights into the infection process.

2019-04-11 | GSE114909 | GEO

<i>Campylobacter jejuni</i> Demonstrates Conserved Proteomic and Transcriptomic Responses When Co-cultured With Human INT 407 and Caco-2 Epithelial Cells.

Project description:Major foodborne bacterial pathogens, such as Campylobacter jejuni, have devised complex strategies to establish and foster intestinal infections. For more than two decades, researchers have used immortalized cell lines derived from human intestinal tissue to dissect C. jejuni-host cell interactions. Known from these studies is that C. jejuni virulence is multifactorial, requiring a coordinated response to produce virulence factors that facilitate host cell interactions. This study was initiated to identify C. jejuni proteins that contribute to adaptation to the host cell environment and cellular invasion. We demonstrated that C. jejuni responds to INT 407 and Caco-2 cells in a similar fashion at the cellular and molecular levels. Active protein synthesis was found to be required for C. jejuni to maximally invade these host cells. Proteomic and transcriptomic approaches were then used to define the protein and gene expression profiles of C. jejuni co-cultured with cells. By focusing on those genes showing increased expression by C. jejuni when co-cultured with epithelial cells, we discovered that C. jejuni quickly adapts to co-culture with epithelial cells by synthesizing gene products that enable it to acquire specific amino acids for growth, scavenge for inorganic molecules including iron, resist reactive oxygen/nitrogen species, and promote host cell interactions. Based on these findings, we selected a subset of the genes involved in chemotaxis and the regulation of flagellar assembly and generated C. jejuni deletion mutants for phenotypic analysis. Binding and internalization assays revealed significant differences in the interaction of C. jejuni chemotaxis and flagellar regulatory mutants. The identification of genes involved in C. jejuni adaptation to culture with host cells provides new insights into the infection process.

| S-EPMC6470190 | biostudies-literature

Proteomics of campylobacter exposed to host Caco-2 and INT407 intestinal cells

Project description:Comparison of Campylobacter proteome in MH media with and without deoxycholic acid, in presence of FBS or after being exposed to INT 407 and Caco2 intestinal epithelial cells.

2019-05-03 | PXD009817 | Pride

Serendipita sp. 407 isolate:407

Project description:Serendipita sp. 407 isolate:407 Genome sequencing and assembly

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The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

Project description:Proteomic data from pulldown performed with the recombinant Campylobacter CiaD-GST fusion was expressed in E. coli BL21(DE3) on a lysate of human cultured INT 407 cells. One non-specific binding control was performed on INT 407 cell lysates and two-pulldowns were realized with the recombinant protein. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline.

2021-01-05 | MSV000086662 | MassIVE

Serendipita sp. 407

Project description:Serendipita sp. 407 Transcriptome - Seb407

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S. flexneri transcriptome in Henle-407 cells

Project description:S. flexneri infected Henle-407 cells were treated with and without 20 mM formate, RNA-seq was used to determine transcriptome changes

2018-09-08 | GSE119622 | GEO

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| PRJNA443925 | ENA

Alcaligenes faecalis strain:407

Project description:Alcaligenes faecalis strain:407 Genome sequencing

| PRJNA1164264 | ENA

The poloxamer P-407 chronic hyperlipidemia mouse model

Project description:We performed a systemic study of the poloxamer P-407-induced chronic hyperlipidemia in C57BL/6J mice. Mice were treated 96 days every third day.The poloxamer mostly elevates plasma triglycerides and cholesterol while change of the liver cholesterol is not significant. Keywords: Disease model state analysis

2006-11-22 | GSE6305 | GEO

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