Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Adipocyte-derived lipids mediate melanoma progression via FATP proteins

ABSTRACT: Adipocyte-derived lipids mediate melanoma progression via FATP proteins

PROVIDER: PRJNA473164 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7221110.fastq.gz	Fastqsanger.gz
	SRR7221111.fastq.gz	Fastqsanger.gz
	SRR7221112.fastq.gz	Fastqsanger.gz
	SRR7221113.fastq.gz	Fastqsanger.gz
	SRR7221114.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Adipocyte-derived lipids mediate melanoma progression via FATP proteins

Project description:We performed RNA-seq on A375 melanoma cells in monoculture or after coculture with adipocytes and identified differentially expressed genes

2018-05-26 | GSE114941 | GEO

Adipocyte-Derived Lipids Mediate Melanoma Progression via FATP Proteins.

Project description:Advanced, metastatic melanomas frequently grow in subcutaneous tissues and portend a poor prognosis. Though subcutaneous tissues are largely composed of adipocytes, the mechanisms by which adipocytes influence melanoma are poorly understood. Using in vitro and in vivo models, we find that adipocytes increase proliferation and invasion of adjacent melanoma cells. Additionally, adipocytes directly transfer lipids to melanoma cells, which alters tumor cell metabolism. Adipocyte-derived lipids are transferred to melanoma cells through the FATP/SLC27A family of lipid transporters expressed on the tumor cell surface. Among the six FATP/SLC27A family members, melanomas significantly overexpress FATP1/SLC27A1. Melanocyte-specific FATP1 expression cooperates with BRAFV600E in transgenic zebrafish to accelerate melanoma development, an effect that is similarly seen in mouse xenograft studies. Pharmacologic blockade of FATPs with the small-molecule inhibitor Lipofermata abrogates lipid transport into melanoma cells and reduces melanoma growth and invasion. These data demonstrate that stromal adipocytes can drive melanoma progression through FATP lipid transporters and represent a new target aimed at interrupting adipocyte-melanoma cross-talk.Significance: We demonstrate that stromal adipocytes are donors of lipids that mediate melanoma progression. Adipocyte-derived lipids are taken up by FATP proteins that are aberrantly expressed in melanoma. Inhibition of FATPs decreases melanoma lipid uptake, invasion, and growth. We provide a mechanism for how stromal adipocytes drive tumor progression and demonstrate a novel microenvironmental therapeutic target. Cancer Discov; 8(8); 1006-25. ©2018 AACR.This article is highlighted in the In This Issue feature, p. 899.

| S-EPMC6192670 | biostudies-literature

Adipocyte Specific Tribbles1 Regulates Plasma Adiponectin and Plasma Lipids in Mice

Project description:The goal of this study is to understand the role of adipocyte-specific Trib1 in adiponectin and plasma lipid regulation. Here, we report that adipocyte-specific Trib1 knockout mice (Trib1_ASKO) have increased plasma adiponectin levels and decreased plasma cholesterol and triglycerides. We demonstrate that loss of Trib1 increases adipocyte production and secretion of adiponectin independent of the known TRIB1 function of regulating proteasomal degradation. RNA-seq analysis of adipocytes and livers from Trib1_ASKO mice suggests that alterations in adipocyte function underlie the plasma lipid changes observed in these mice. Secretomics and RNA-seq analysis revealed that Trib1_ASKO mice have increased production of Lpl and decreased production of Angptl4 in adipose tissue, and fluorescent substrate assays confirm an increase in adipose tissue Lpl activity, which likely underlies the observed triglyceride phenotype. In summary, we demonstrate here a novel role for adipocyte Trib1 in regulating plasma adiponectin, total cholesterol, and triglycerides in mice, confirming previous genetic associations observed in humans and providing a novel avenue through which Trib1 regulates plasma lipids and coronary artery disease.

2022-06-30 | GSE168596 | GEO

Adipocyte Specific Tribbles1 Regulates Plasma Adiponectin and Plasma Lipids in Mice

Project description:Adipocyte Specific Tribbles1 Regulates Plasma Adiponectin and Plasma Lipids in Mice

| PRJNA708349 | ENA

Abundances of transcripts, proteins, metabolites, and lipids in the cell cycle of budding yeast

Project description:For the first time in any system, we generated experiment-matched datasets of the levels of RNAs, proteins, metabolites, and lipids from un-arrested, growing, and synchronously dividing yeast cells.

2019-12-11 | GSE135476 | GEO

Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamics

Project description:Extracellular vesicles are emerging key actors in adipocyte communication. Notably, small extracellular vesicles shed by adipocytes promote melanoma aggressiveness through fatty acid oxidation, with a heightened effect in obesity. However, the vesicular actors and cellular processes involved remain largely unknown. Here, we elucidate the mechanisms linking adipocyte extracellular vesicles to metabolic remodeling and cell migration. Using an adapted SILAC technique, we show that adipocyte vesicles stimulate melanoma fatty acid oxidation by providing both enzymes and substrates. In obesity, the heightened effect of extracellular vesicles depends on increased transport of fatty acids, not fatty acid oxidation related enzymes as shown by classical LC-MS/MS analysis. These fatty acids, stored within lipid droplets in cancer cells, drive fatty acid oxidation after release through lipophagy. This increase in mitochondrial activity redistributes mitochondria to membrane protrusions of migrating cells, which is necessary to increase cell migration in the presence of adipocyte vesicles. Our results provide key insights into the role of extracellular vesicles in the metabolic cooperation that takes place between adipocytes and tumors with particular relevance in obesity.

2020-01-09 | PXD014487 | Pride

Abundances of transcripts, proteins, metabolites, and lipids in the cell cycle of budding yeast

Project description:Abundances of transcripts, proteins, metabolites, and lipids in the cell cycle of budding yeast

| PRJNA559005 | ENA

MHC class II proteins mediate cross-species entry of bat influenza viruses

Project description:MHC class II proteins mediate cross-species entry of bat influenza viruses

| PRJNA803440 | ENA

Postnatal lipids drive hepatocyte maturation

Project description:Postnatal lipids drive hepatocyte maturation

| PRJNA559823 | ENA

STAG proteins mediate heterochromatin organization to support translation and cell identity [RNA-Seq]

Project description:STAG proteins mediate heterochromatin organization to support translation and cell identity [RNA-Seq]