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Efficient C-to-T base editing in methylated region with human APOBEC3A

ABSTRACT: Efficient C-to-T base editing in methylated region with human APOBEC3A

PROVIDER: PRJNA473503 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7228040_1.fastq.gz	Fastqsanger.gz
	SRR7228040_2.fastq.gz	Fastqsanger.gz
	SRR7228050_1.fastq.gz	Fastqsanger.gz
	SRR7228050_2.fastq.gz	Fastqsanger.gz
	SRR7228060_1.fastq.gz	Fastqsanger.gz

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Efficient C-to-T base editing in methylated region with human APOBEC3A

Project description:Base editors (BEs) shed new light on correcting disease-related T-to-C mutations. However, current rat APOBEC1-based BEs are less efficient in editing cytosines in highly-methylated regions or in GpC context. By screening a variety of APOBEC/AID deaminases, we showed that human APOBEC3A-conjugated BE and its engineered forms can mediate efficient C-to-T base editing in all examined contexts, including regions with high-methylation levels and GpC dinucleotides, which extends base editing scope.

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