Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Deep sequencing analyzed the coverage of sgRNA in haploid stem cells.

ABSTRACT: Deep sequencing analyzed the coverage of sgRNA in haploid stem cells.

PROVIDER: PRJNA473519 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7229558_1.fastq.gz	Fastqsanger.gz
	SRR7229558_2.fastq.gz	Fastqsanger.gz
	SRR7229559_1.fastq.gz	Fastqsanger.gz
	SRR7229559_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Deep sequencing analyzed the coverage of sgRNA in haploid stem cells.

Project description:We used the deep sequencing to analyze the sgRNA coverage of Dnd1 library in 4B2N1-DP1 and 4B2N1-DP2 cell lines. The amplicon obtained from PCR reaction from genome using high-fidelity DNA polymerase. By obtaining over 10 million reads of each sample from deep sequencing, we mapped the reads to the reference sequence and then calculated coverage and counts. The results showed that the hit number of different sgRNAs were variable

2018-08-06 | GSE115015 | GEO

Responses to ethanol in haploid and diploid strain

2010-01-30 | GSE20108 | GEO

Responses to ethanol in haploid and diploid strain

Project description:Diploid and haploid strains often exhibit different tolerance to variety of stresses. Transcriptome of acclimation to ethanol stress in diploid and haploid strain of Saccharomyces cerevisiae was analyzed. We analyzed transcriptome profiles of diploid and haploid strains in the presence of ethanol. Haploid and diploid strains were cultured in YEPD media with 0%, 3% and 7% ethanol(v/v) in fermentors. The samples were collected at the growth stage for each strain under different conditions.

2010-02-21 | E-GEOD-20108 | biostudies-arrayexpress

Transcriptome responses to sublethal concentration of abamectin in Tetranychus truncatus analyzed by deep-sequencing

Project description:Transcriptome responses to sublethal concentration of abamectin in Tetranychus truncatus analyzed by deep-sequencing

| PRJNA475023 | ENA

Analysis of Cas9/sgRNA mutagenic activity at a variety of loci in zebrafish (Run6)

Project description:Analysis of Cas9/sgRNA mutagenic activity at a variety of loci in zebrafish. Each loci has a control, where no Cas9/sgRNA were injected. This is amplicon sequencing with Illumina, after PCR amplification. Data was processed with ampliCan R package version 1.1.1.

2017-12-25 | E-MTAB-6310 | biostudies-arrayexpress

Derivation and differentiation of haploid human embryonic stem cells [methylation array]

Project description:Diploidy is a fundamental genetic feature in mammals, in which haploid cells normally arise only as post-meiotic germ cells that serve to insure a diploid genome upon fertilization. Gamete manipulation has yielded haploid embryonic stem (ES) cells from several mammalian species, but as of yet not from humans. Here we analyzed a large collection of human parthenogenetic ES cell lines originating from haploid oocytes, leading to the successful isolation and maintenance of human ES cell lines with a normal haploid karyotype. Haploid human ES cells exhibited typical pluripotent stem cell characteristics such as self-renewal capacity and a pluripotency-specific molecular signature. Although haploid human ES cells resembled their diploid counterparts, they also displayed distinct properties including differential regulation of X chromosome inactivation and genes involved in oxidative phosphorylation, alongside reduction in absolute gene expression levels and cell size. Intriguingly, we found that a haploid genome is compatible not only with the undifferentiated pluripotent state, but also with differentiated somatic fates representing all three embryonic germ layers, despite a persistent dosage imbalance between the autosomes and X chromosome. We expect that haploid human ES cells will provide novel means for studying human functional genomics, development and evolution.

2016-03-16 | GSE71457 | GEO

Derivation and differentiation of haploid human embryonic stem cells [SNP array]

2016-03-16 | GSE74950 | GEO

Derivation and differentiation of haploid human embryonic stem cells [expression array]

2016-03-16 | GSE74805 | GEO

Analysis of Cas9/sgRNA mutagenic activity at a variety of loci in zebrafish (Run 9)

2017-12-15 | E-MTAB-6357 | biostudies-arrayexpress

Analysis of Cas9/sgRNA mutagenic activity at a variety of loci in zebrafish (Run 10)

2017-12-15 | E-MTAB-6358 | biostudies-arrayexpress