Small RNAs deep sequencing from sweet potato in wounding stress
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ABSTRACT:
PROVIDER: PRJNA474012 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR7244047.fastq.gz | Fastqsanger.gz | |||
| SRR7244048.fastq.gz | Fastqsanger.gz |
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Project description:Plant microRNAs (miRNAs) have emerged as important regulators in developmental processes and stress responses in plants. To identify the wound-responsive miRNAs in the leaves of sweet potato, small RNA deep sequencing was conducted on unwounded and wounded leaves (30 min). Total RNAs were isolated for library construction and analyzed by RNA-sequencing via Illumina Genome Analyzer IIx platform. About 16 million total reads were obtained for each sample.
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Project description:Small RNAs (sRNAs) play important roles in plants encountering stress environments. However, limited research has been conducted on the sRNAs involved in plant wound responses. To identify potential roles for the wounding-related sRNAs, sRNA deep sequencing was used. After leaves were wounded for 0.5 hour, total RNAs from unwounded and wounded leaves were isolated for sRNA library construction. The Illumina platform was used to sequence sRNA libraries. About 12 million sequence reads were obtained for each sample.
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Project description:MicroRNAs (miRNAs) control their target genes by RNA cleavage or translation inhibition. The regulation of miRNA in the local leaves of plants after wounding has been studied, but its systemic effect is less understood. In this study, wounding-induced miR168-3p in the systemic leaves of sweet potato (Ipomoea batatas cv. Tainung 57) was analyzed. Bioinformatics analysis indicated that the target gene of miR168-3p is IbKTN80, which encodes the katanin p80 subunit. Their relationship was confirmed by agroinfiltration with transient expression of primary miR168 and IbKTN80 transcript. Transgenic sweet potatoes overexpressing and silencing miR168-3p further confirmed that IbKTN80 was the target gene of miR168-3p. Moreover, IbKTN80 is homologous to AtKTN80.2, and the expression of IbKTN80 could recover the functional defectiveness of the AtKTN80.2 in Arabidopsis treated with abscisic acid. IbKTN80, similar to AtKTN80.2, regulated MYC2, which is involved in the jasmonate (JA) signaling and biosynthesis. Conclusively, wounding induced the systemic generation of miR168-3p, which repressed the expression of IbKTN80. The miR168-3p-IbKTN80 module was involved in JA regulation and may participate in the signal transduction of systemic effect.
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