Acute effects of active breaks during prolonged sitting on subcutaneous adipose tissue gene expression
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA475673 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Breaking up prolonged periods of time spent sitting has a range of beneficial impacts on cardiometabolic risk biomarkers. The molecular mechanisms include regulation of skeletal muscle gene and protein expression controlling metabolic, inflammatory and cell development pathways. An active communication network exists between adipose and muscle tissue, but the effect of active breaks in prolonged sitting on adipose tissue have yet to be investigated. This study characterised the acute transcriptional events induced in adipose tissue by regular active breaks during prolonged sitting. In a subset of 8 overweight/obese adults participating in an acute randomised three-intervention crossover trial, subcutaneous adipose tissue biopsies were obtained after each condition. The three experimental conditions were conducted in the postprandial state and included: i) prolonged uninterrupted sitting; or prolonged sitting interrupted with 2-minute bouts of ii) light- or iii) moderate-intensity treadmill walking every 20 minutes. Microarrays identified 36 differentially expressed genes between the three conditions (fold change≥0.5 in either direction; p<0.05). Pathway analysis indicated that breaking up of prolonged sitting led to differential regulation of adipose tissue metabolic networks and inflammatory pathways, increased insulin signalling, increased adipocyte turnover, and facilitated cross-talk between adipose tissue and other organs. This study provides insight into the adipose tissue regulatory systems and transcriptional processes that contribute to the physiological benefits of interrupting prolonged sitting.
2018-06-13 | GSE115645 | GEO
Project description:To investigate the proteomic profiles of paired subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) samples, as well as their correlations with clinical traits in severely obese patients, and to identify potential serum protein markers associated with tissue expression or metabolic states.
2023-10-16 | PXD041721 | Pride
Project description:Adipose, once considered an inert storage depot, is now known to be an active endocrine tissue involved in total body homeostasis and metabolism, which exerts effects on multiple systems including food intake, immune function, and blood glucose regulation. During the development of obesity, adipose undergoes major expansion and remodeling, but the biological processes involved in this transition are not well understood. Thus, we used canine microarrays to analyze gene expression profiles of subcutaneous adipose tissue during the transition from a lean to obese phenotype. Subcutaneous adipose tissue samples were collected from 9 intact female beagles (4 yr-old; 4 control; 5 ad libitum) at baseline, 4, 8, 12 and 24 wk after beginning ad libitum feeding.
2010-09-14 | E-GEOD-24099 | biostudies-arrayexpress
Project description:To investigate the proteomic profiles of paired subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) samples, as well as their correlations with clinical traits in severely obese patients, and to identify potential serum protein markers associated with tissue expression or metabolic states.
2024-05-24 | PXD041750 | Pride
Project description:In addition to total body fat, the regional distribution and inflammatory status of enlarged adipose tissue are strongly linked to metabolic and cardiovascular complications of obesity. We recently showed that the severity of liver non-alcoholic histopathology in obese subjects increased with the amounts of macrophages in visceral adipose tissue (VAT), while no relation was found with the subcutaneous adipose tissue (SAT). In the abdominal region, SAT is anatomically divided into superficial (sSAT) and deep (dSAT) layers. The aim of the present study was to test the hypothesis that these distinct compartments differentially contribute to hepatic alteration in obesity. Total RNA was isolated from two different strata of human adipose tissue of 8 subjects.
2011-08-31 | E-GEOD-30133 | biostudies-arrayexpress
Project description:To identify genes differentially expressed in abdominal and gluteal adipose tissue, we determined the mRNA transcription profile of 10 abdominal and 10 gluteal female adipose tissue sections using Agilent Whole Human Genome Microarrays. RNA of 10 subcutaneous abdominal and 10 subcutaneous gluteal fat depot samples was processed by Miltenyi Biotech GmbH (Bergisch Gladbach, Germany) and loaded on single-color Whole Human Genome 4x44K microarrays (G4112F) from Agilent Technologies.
2013-11-15 | E-GEOD-47511 | biostudies-arrayexpress
Project description:Adipose, once considered an inert storage depot, is now known to be an active endocrine tissue involved in total body homeostasis and metabolism, which exerts effects on multiple systems including food intake, immune function, and blood glucose regulation. Adipose tissue depots are known to have unique metabolic and gene expression profiles in vivo and when cultured in vitro. Differences in adipose tissue depot function could be important in determining chronic disease risk. Few comparisons of depot gene expression have been performed in the dog. Utilizing microarray technology, our objective was to identify differentially expressed genes and enriched functional pathways between subcutaneous and gonadal adipose of lean and obese dogs. Subcutaneous and gonadal adipose tissue samples were collected from 9 intact female beagles (4 yr-old; 4 lean controls; 5 obese ad libitum-fed) after 24 wk of ad libitum feeding.
2012-01-15 | E-GEOD-29280 | biostudies-arrayexpress
Project description:In addition to total body fat, the regional distribution and inflammatory status of enlarged adipose tissue are strongly linked to metabolic and cardiovascular complications of obesity. We recently showed that the severity of liver non-alcoholic histopathology in obese subjects increased with the amounts of macrophages in visceral adipose tissue (VAT), while no relation was found with the subcutaneous adipose tissue (SAT). In the abdominal region, SAT is anatomically divided into superficial (sSAT) and deep (dSAT) layers. The aim of the present study was to test the hypothesis that these distinct compartments differentially contribute to hepatic alteration in obesity.
2011-09-01 | GSE30133 | GEO
Project description:human paired adipose tissue (subcutaneous and omentum) before and after gastric-bypass and weight loss
2014-12-09 | E-MTAB-3052 | biostudies-arrayexpress
Project description:Previous publications demonstrated that there were anatomical, physiological, cellular, clinical and prognostic differences among adipose tissue present in subcutaneous areas, abdominal cavity and outside adventitial layer of artery. Thus, we wondered whether ASCs from subcutaneous adipose tissue, abdominal adipose tissue and perivascular adipose tissue were also different in gene expression. Here we performed bulk RNA-Seq assay for subcutaneous, abdominal and perivascular adipose derived stem cells.
2021-04-13 | GSE171946 | GEO