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Microglial gene expression after TGFb1 treatment in vitro

ABSTRACT: Microglial gene expression after TGFb1 treatment in vitro

PROVIDER: PRJNA475680 | ENA |

REPOSITORIES: ENA

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Microglial gene expression after TGFb1 treatment in vitro

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RNA-seq of LPS-challenged microglial cell treatment with TOP1 inhibitor

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Radial glia promote microglial development through aVb8 -TGFb1 signaling [ChIP-seq]

Project description:In this study, we aimed to identify 1) The relevant Itgb8 expressing cell types that mediate microglial TGFb activation; 2) The developmental timing of Itgb8-mediated TGFb signaling in microglia; 3) The cellular source and identity of the TGFb ligand relevant for microglial development and homeostasis; 4) The relationship between developmentally disrupted microglia and disease associated microglia; and 5) The role of canonical (Smad-mediated) versus non-canonical TGFb signaling in microglia.

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LPS and Telmisartan co-treatment of microglial BV2 cells

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Effect of TGFb1 treatment and WISP1 overexpression on 4T1 breast cancer cells

Project description:This study set out to identify TGFb1 transcriptional targets in breast cancer cells. 4T1 breast cancer cells were treated with TGFb1 for 24h and transcripts that were significantly regulated were identified. Wisp1 was identified as one of the genes most highly upregulated by TGFb1. To identify the transcriptional targets of Wisp1, 4T1 cells were then virally transduced to increase Wisp1 expression. The gene expression profiles of Wisp1-overexpressing 4T1 cells were determined and revealed that Wisp1 overexpression does not result in major gene expression changes in 4T1 cells.

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