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Transcriptomic data of MCF7 cells adapted to culture in media containing different sugars (glucose or fructose) and cultured in 2D on plastic

ABSTRACT: Transcriptomic data of MCF7 cells adapted to culture in media containing different sugars (glucose or fructose) and cultured in 2D on plastic

PROVIDER: PRJNA476336 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7348437_1.fastq.gz	Fastqsanger.gz
	SRR7348437_2.fastq.gz	Fastqsanger.gz
	SRR7348438_1.fastq.gz	Fastqsanger.gz
	SRR7348438_2.fastq.gz	Fastqsanger.gz

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Transcriptomic data of MCF7 cells adapted to culture in media containing different sugars (glucose or fructose) and cultured in 2D on plastic

Project description:We report the single-cell RNA sequencing data obtained from MCF7 breast cancer cells cultured in standard DMEM with 25 mM glucose, or adapted to culture in DMEM with 10 mM fructose to reduce glycolysis, and cultures in 2D on plastic

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A Pilot Study to Determine Fructose Uptake by Primary Human Colorectal Tumors

Project description:This proposed study is designed to investigate the specific uptake of fructose by human colorectal tumors. In this study, subjects with colorectal cancer undergoing surgery will receive an oral sugar solution containing fructose or xylose prior to surgery. The tumor will then be resected, and a portion of the tissue will be used to measure the abundance of fructose and xylose. The study hypothesis is that the tumors will take up fructose sugar but not xylose sugar. A comparison of the sugar uptake between the tumor and normal tissues from the adjacent intestinal epithelium and smooth muscle and the liver will be conducted. This proposal will confirm that human colorectal cancer tumors can directly absorb dietary sugars, which has never been demonstrated.

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Transcriptomic data of MDA-MB-231 cells adapted to culture in media containing different sugars (glucose or fructose) and cultured as mammospheres

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High-Fructose Corn Syrup Enhances Intestinal Tumor Growth in Mice

Project description:Excessive consumption of beverages sweetened with high-fructose corn syrup (HFCS) is associated with obesity and with an increased risk of colorectal cancer. Whether HFCS contributes directly to tumorigenesis is unclear. We investigated the effects of daily oral administration of HFCS in APC mutant mice, which are predisposed to develop intestinal tumors. The HFCS-treated mice showed a dramatic increase in tumor size and tumor grade in the absence of obesity or metabolic syndrome. HFCS increased the levels of fructose and glucose in the intestinal lumen and serum, respectively, and the tumors absorbed both sugars. Within the tumors, fructose was converted to fructose-1-phosphate, leading to activation of glycolysis and increased synthesis of fatty acids that support tumor growth. These mouse studies support the hypothesis that the combination of dietary glucose and fructose, even at a moderate dose, can enhance tumorigenesis.

2019-03-29 | GSE126858 | GEO

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Project description:Comparison of gene expression data between cell lines grown on 2D tissue culture plastic or 3D matrigel A427, A549, H23, H358, H460, H661, H1437, and H1703 cells were compared

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Effect of Integrin-aBb2 on transcription in 2d or 3d.

Project description:M21 or M21L cells were grown either in 2d (on plastic) or in a 3d-collagen model. 2x2 design, in triplicate

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Transcriptomic data of MDA-MB-231 cells adapted to culture in media containing different sugars (glucose or fructose) and cultured as mammospheres

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