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Single-cell ID-seq identifies BMP signaling as a driver of a late stage epidermal differentiation program

ABSTRACT: Single-cell ID-seq identifies BMP signaling as a driver of a late stage epidermal differentiation program

PROVIDER: PRJNA476535 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7353040.fastq.gz	Fastqsanger.gz
	SRR7353041.fastq.gz	Fastqsanger.gz
	SRR7353042.fastq.gz	Fastqsanger.gz
	SRR7353043.fastq.gz	Fastqsanger.gz
	SRR7353034.fastq.gz	Fastqsanger.gz

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Single-cell ID-seq identifies BMP signaling as a driver of a late stage epidermal differentiation program.

Project description:Single-cell ID-seq identifies BMP signaling as a driver of a late stage epidermal differentiation program.

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Single-cell ID-seq identifies BMP signaling as a driver of a late stage epidermal differentiation program

Project description:We developed a single cell application of the sequencing-based immunodetection method ID-seq which makes it possible to multiplex measurements of many different epitopes from proteins and phosphoproteins in a high throughput setting. A FACS sort step was implemented to allow sorting of single cells in 96 well plates. With these DNA-tagged antibodies and dedicated sample preparation protocol adapted for single cells, we studied the studied the changes of 70 (phospho-)proteins in primary human skin cells compared to a subpopulation with low ITGB1 protein expression. Given that ITGB1 is a skin stem cell marker, we indeed found that the ITGB1 low cells are differentiated and we found a role for BMP signaling in this process.

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Single-cell ID-seq identifies BMP signaling as a driver of a late stage epidermal differentiation program.

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Identification of bone morphogenetic protein (BMP)-7 as a key instructive factor for human epidermal Langerhans cell differentiation and proliferation

Project description:Langerhans dendritic cells represent abundantly occuring and evolutionary highly conserved DCs specifically located in the stratified epithelial tissues. LCs are unique among DC family members in that they express epithelial-type adhesion molecules, allowing them to form a tight three-dimensional network in basal and suprabasal epidermal keratinocyte layers and developmentally dependent on the cytokine TGF-M-NM-21. In the present study, we identified BMP-7 as another key factor inducing LC differnetiation. Here we have performed comparative analysis of highly purified CD207+/CD1a+ in vitro generated Langerhans cells in the presence of BMP-7 and TGF-M-NM-21. We have identified that both BMP-7-LCs and TGF-M-NM-21-LCs are closely related to each other. CD34+ hematopoietic stem cells were isolated from freshly obtained cord blood and cultured in the presence of GM-CSF, SCF, Flt3L, TNFa and either TGF-M-NM-21 or BMP-7 for 7 days to get immature LCs. RNA was isolated from these in vitro genetared LCs.

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Identification of bone morphogenetic protein (BMP)-7 as a key instructive factor for human epidermal Langerhans cell differentiation and proliferation

Project description:Langerhans dendritic cells represent abundantly occuring and evolutionary highly conserved DCs specifically located in the stratified epithelial tissues. LCs are unique among DC family members in that they express epithelial-type adhesion molecules, allowing them to form a tight three-dimensional network in basal and suprabasal epidermal keratinocyte layers and developmentally dependent on the cytokine TGF-β1. In the present study, we identified BMP-7 as another key factor inducing LC differnetiation. Here we have performed comparative analysis of highly purified CD207+/CD1a+ in vitro generated Langerhans cells in the presence of BMP-7 and TGF-β1. We have identified that both BMP-7-LCs and TGF-β1-LCs are closely related to each other.

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