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TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [TET2 ChIP-seq ES cells]

ABSTRACT: TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [TET2 ChIP-seq ES cells]

PROVIDER: PRJNA476604 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7359630.fastq.gz	Fastqsanger.gz
	SRR7359631.fastq.gz	Fastqsanger.gz
	SRR7359632.fastq.gz	Fastqsanger.gz
	SRR7359633.fastq.gz	Fastqsanger.gz
	SRR7359624.fastq.gz	Fastqsanger.gz

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TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [TET2 ChIP-seq ES cells]

Project description:The epigenetic regulator TET2 is frequently mutated in hematological diseases. Mutations have been shown to arise in hematopoietic stem cells early in disease development, lead to altered DNA methylation landscapes and to an increased risk of hematopoietic malignancy. Here, we show by genome-wide mapping of TET2 binding sites in different cell types that TET2 localizes to regions of open chromatin and cell-type specific enhancers. We find that deletion of Tet2 in native hematopoiesis as well as fully transformed Acute Myeloid Leukemia (AML) results in changes in transcription factor (TF) activity within these regions, and we provide evidence that loss of TET2 leads to attenuation of chromatin binding of members of the basic helix-loop-helix (bHLH) TF family. Together, these findings demonstrate that TET2 activity shapes the local chromatin environment at enhancers to facilitate TF binding and provide a compelling example of how epigenetic dysregulation can affect gene expression patterns and drive disease development.

2019-01-22 | GSE115964 | GEO

Tet2 regulates Sin3a recruitment at active enhancers in embryonic stem cells

Project description:Tet2 regulates Sin3a recruitment at active enhancers in embryonic stem cells

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TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells

Project description:TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells

| PRJNA476603 | ENA

TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [TET2 ChIP-seq hematopoietic cells]

Project description:TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [TET2 ChIP-seq hematopoietic cells]

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Tet2 regulates Sin3a recruitment at active enhancers in embryonic stem cells

Project description:In this study: (1) we distinguished Tet2 target genes that are regulated by its catalytic vs. noncatalytic functions in ESCs by transcriptomic profiling of Tet2 wildtype (WT), Tet2 catalytic mutant (Mut), and Tet2 knockout (KO) mouse ESCs by RNA-seq. (2) We mapped genome-wide DNA methylation of Tet2-WT, Tet2-Mut, and Tet2-KO ESCs by WGBS, to establish a critical role for Tet2 in demethylating promoters and enhancers of its catalytic target genes. (3) We determined how the genome-wide occupancy of the epigenetic modifiers Sin3a and Sap30 and the enrichment of H3K27ac, are affected in Tet2-Mut and Tet2-KO ESCs versus Tet2-WT by CUT&Tag and identified that Tet2 deficiency diminishes Sin3a occupancy at promoters and enhancers. (4) We mapped Sin3a levels genome-wide in Tet1/2 double catalytic mutant (DMUT) and Tet1/2 double knockout (DKO) ESCs, and found that deficiency of both Tet1 and Tet2 resulted in decreased levels of Sin3a in a subset of active enhancers.

2023-06-23 | GSE213398 | GEO

TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [RNA-Seq]

Project description:TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [RNA-Seq]

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TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [ATAC-Seq]

Project description:TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [ATAC-Seq]

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TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [TET2 ChIP-seq hematopoietic cells]

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TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [ATAC-Seq]

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TET2 binding to enhancers facilitates transcription factor recruitment in hematopoietic cells [RNA-Seq]