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The Long Noncoding RNA Lncenc1 Regulates Glycolysis in Naive Mouse ES Cells I

ABSTRACT: The Long Noncoding RNA Lncenc1 Regulates Glycolysis in Naive Mouse ES Cells I

PROVIDER: PRJNA478566 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7456640_1.fastq.gz	Fastqsanger.gz
	SRR7456640_2.fastq.gz	Fastqsanger.gz
	SRR7456641_1.fastq.gz	Fastqsanger.gz
	SRR7456641_2.fastq.gz	Fastqsanger.gz
	SRR7456642_1.fastq.gz	Fastqsanger.gz

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The Long Noncoding RNA Lncenc1 Regulates Glycolysis in Naive Mouse ES Cells

Project description:The Long Noncoding RNA Lncenc1 Regulates Glycolysis in Naive Mouse ES Cells

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The Long Noncoding RNA Lncenc1 Regulates Glycolysis in Naive Mouse ES Cells II

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The Long Noncoding RNA Lncenc1 Regulates Glycolysis in Naive Mouse ES Cells II

Project description:The naive embryonic stem cells (nESCs) display unique characteristics mirroring naive pluripotency in vivo, but the molecular mechanisms underlying the self-renewal of nESCs remain incompletely understood. Here we analyzed stranded transcriptomes in mouse nESCs and epiblast-like cells (EpiLCs), and identified 135 long non-coding RNAs (lncRNAs) preferentially expressed in nESCs. We further investigated the functions of Lncenc1, a highly abundant lncRNA in mouse nESCs. Knockdown or knockout of Lncenc1 in mouse nESCs leads to significantly decreased expression of core pluripotency genes and significant reduction of colony formation capability. Furthermore, depletion of Lncenc1 down-regulates the glycolysis pathway, as indicated by significant decreases of glycolysis genes expression, glucose consumption, lactate production and Seahorse data. Mechanically, Lncenc1 associates with RNA-binding proteins PTBP1 and HNRNPK functionally. Together, we demonstrate that Lncenc1 regulates the glycolysis in mouse nESCs, suggesting novel functions of lncRNAs in linking energy metabolism and pluripotent stem cells.

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The Long Noncoding RNA Lncenc1 Regulates Glycolysis in Naive Mouse ES Cells I

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The Long Noncoding RNA Lncenc1 Regulates Glycolysis in Naive Mouse ES Cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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RNA-seq of naive and primed ES cells

Project description:Here we propose a set of molecular criteria for evaluating the naive human pluripotent state. We show by RNA-seq that transcription of transposable elements provides a sensitive measure of the concordance between pluripotent stem cells and early human development. RNA-seq of 4 naive ES samples in 4i/LA, 3 naive ES samples in 5i/LA, 2 transgene-dependant naive ES cell samples, and 5 primed ES cell samples (in hESM)

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Project description:1. Evaluate the diagnostic value of long noncoding RNA (CCAT1) expression by RT-PCR in peripheral blood in colorectal cancer patients versus normal healthy control personal. 2. Evaluate the clinical utility of detecting long noncoding RNA (CCAT1) expression in diagnosis of colorectal cancer patients & its relation to tumor staging. 3. Evaluate the clinical utility of detecting long noncoding RNA (CCAT1) expression in precancerous colorectal diseases. 4. Compare long noncoding RNA (CCAT1) expression with traditional marker; carcinoembryonic antigen (CEA) and Carbohydrate antigen 19-9 (CA19-9) in diagnosis of colorectal cancer.

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