Project description:Salt responsive genes were identified in chinese willow (Salix matsudana) after the plants were treated with 100 mM NaCl. for 48 hours We used microarrays to identify genes responsible for combating salt stress. Those up-regulated during the NaCl treatment may protect the plants from damages caused by salt stress.
Project description:Salt responsive genes were identified in chinese willow (Salix matsudana) after the plants were treated with 100 mM NaCl. for 48 hours We used microarrays to identify genes responsible for combating salt stress. Those up-regulated during the NaCl treatment may protect the plants from damages caused by salt stress. 2 month-old S. matsudana plants which were treated with 100 mM NaCl and control plants were used for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain salt responsive genes that protect the plants from stress injury.Those differentially expressed genes identified by the microarray would help to understand the mechanism of S. matsudana reacting to salt stress.
Project description:In order to screening the responsive miRNAs and target genes of willow under salt stress, the 30-day-old plants were exposed to the salt solution (100 mmol L-1 NaCl) for 0 h and 2 d. then RNA isolated from root and stem tissues for the same time point were mixed respectively in equal amounts for small RNA (sRNA) sequencing. sRNAs with 16–30 nt were separated from 1 µg total RNA by size fractionation. Subsequently, the selected sRNA fragments were ligated with specialized adaptors to the 5’ and 3’ ends (Illumina) using T4 RNA ligase. Then, the ligated RNAs were reverse transcribed and amplified for sequencing using Illumina Hiseq2500 (LC Sciences, Hangzhou, China). Salt stress-responsive miRNAs were identified by comparing the expression levels of miRNAs between the two libraries. Equal amounts of all 2 RNA samples were mixed together to construct one degradome library, and then sent to Hangzhou LC-Bio Co., Ltd (Hangzhou, China) for sequencing by Illumina Genome Analyzer GA-I (Illumina, San Diego, CA, USA).