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Expression profile of Pseudomonas putida KT2440 growing in myristic acid

ABSTRACT: Expression profile of Pseudomonas putida KT2440 growing in myristic acid

PROVIDER: PRJNA480068 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7495270_1.fastq.gz	Fastqsanger.gz
	SRR7495270_2.fastq.gz	Fastqsanger.gz
	SRR7495271_1.fastq.gz	Fastqsanger.gz
	SRR7495271_2.fastq.gz	Fastqsanger.gz
	SRR7495272_1.fastq.gz	Fastqsanger.gz

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Expression profile of Pseudomonas putida KT2440 growing in myristic acid

Project description:We report the application of a high-throughput technique, RNA-seq, to study the transcriptomic pattern of P. putida KT2440 cultures using myristic acid as the sole carbon source at the exponential phase of growth compared with cultures grown with glucose as the sole carbon source. Thus, we can explore the expression of genes involved in fatty acid degradation.

2018-12-31 | GSE116756 | GEO

Transcription profiling by array of Pseudomonas putida KT2440 during exponential phase growing in minimal medium with or without naphthalene

Project description:Transcriptional profile of Pseudomonas putida KT2440 during exponential phase growing in minimal medium saturated with naphthalene compared with the same strain in naphthalene-free medium.

2010-11-01 | E-MEXP-2823 | biostudies-arrayexpress

Gene expression of P. putida KT2440 cultivated in an anoxic bioelectrochemical system

Project description:To get insights in the electrogenic anaerobic lifestyle of P. putida KT2440 cultivated in a bioelectrochemical system (BES), we employed whole genome microarray expression profile.

2024-09-18 | GSE266590 | GEO

Pseudomonas putida KT2440: Control vs. indole 1mM treated

Project description:Transcriptional profiling of P. putida KT2440 cells comparing control untreated cells with 1 mM indole treated cells

2012-10-02 | GSE41254 | GEO

Expression data from Pseudomonas putida KT2440 Tn5 mutants

Project description:We used Progenika oligonucleotide arrays to monitor the gene expression after cold shock from 30°C to 10°C. The 10°C samples of the P. putida wild type were compared to those of the respective P. putida KT2440 Tn5 mutants affected in either cbrA (PP4695), cbrB (PP4696), pcnB (PP4697), vacB (PP4880) or bipA (PP5044).

2014-02-01 | GSE28257 | GEO

Generation of Ionic Liquid Tolerant Pseudomonas putida KT2440 Strains via Adaptive Laboratory Evolution

Project description:Transcriptome of the wildtype or evolved Pseudomonas putida KT2440

2020-07-08 | GSE149827 | GEO

Transcriptome of Pseudomonas putida KT2440 in response to selenite

Project description:The bacterium Pseudomonas putida KT2440 has the ability to reduce selenite forming nanoparticles of elemental selenium. This is the transcriptome of the organism when cultured in the presence of selenite.

2022-10-01 | GSE214391 | GEO

Pseudomonas putida KT2440 : Control vs. paraquat (PQ) or cumen hydroperoxide (CHP) treated

Project description:Transcriptional profiling of P. putida KT2440 cells comparing control untreated cells with PQ or CHP treated cells

2011-12-15 | GSE34409 | GEO

The effect of multiple phaJ deletions Pseudomonas putida KT2440 on polyhydroxyalkanoates synthesis

Project description:Pseudomonas putida KT2440 is a well-known model organism for the medium chain length (mcl) PHA accumulation. (R)-Specific enoyl-coenzyme A hydratase (PhaJ) was considered to be the main supplier of monomers for PHA synthesis by converting the -oxidation intermediate, trans-2-enoyl-CoA to (R)-3-hydroxyacyl-CoA when fatty acids (FA) are used. Three PhaJ homologues, PhaJ1, PhaJ4 and MaoC are annotated in P. putida KT2440. To investigate the relationship of fatty acids - PHA metabolism and the role of each PhaJ in PHA biosynthesis in P. putida KT2440, a series of P. putida KT2440 knockouts was obtained. PHA content and monomer composition in WT and mutants under different growth conditions were analysed. However, when all three PhaJ homologues were deleted, the mutant still accumulated PHA up to 10.7 % of the cell dry weight (CDW). To identify other potential PHA monomer suppliers by analysing the proteome of the phaJ1maoCphaJ4. The proteomes of the WT, phaJ1phaJ4 and phaJ1maoCphaJ4 strains in MSM medium with octanoate under nitrogen limited condition were detected. In addition, we found that the deletion of PhaJ1 in P. putida KT2440 has a negative impact on the PHA accumulation in cells cultivated on glucose with nitrogen limitation conditions. It seems PhaJ1 also mediates the synthesis of PHA when glucose was used as the carbon and energy source. To investigate the role of PhaJ1 in PHA accumulation with glucose, the proteomes of P. putida KT2440 wild type, phaJ1, phaJ1phaJ4 and phaJ1maoCphaJ4 mutant growing on glucose were detected and compared.

2023-05-10 | PXD037932 | Pride

Transcription profiling by array of Pseudomonas putida KT2440 grown with or without chloramphenicol

Project description:Transcriptional profile of Pseudomonas putida KT2440 grown in the presence of chloramphenicol (25 mg/L) compared with the same cells grown in absence of chloramphenicol.

2010-11-01 | E-MEXP-2831 | biostudies-arrayexpress

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