Project description:Observational studies in human suggest involvement of vitamin A/retinoic acid (RA) signaling in the regulation of airway smooth muscle (ASM) function, but the precise mechanisms by which RA impacts ASM phenotype is not clear. Here, we generated trascriptional profiles from two different models of RA-sufficient and RA-deficient mouse ASM in order to determine the molecular targets of RA in ASM (VAS/VAD, CTR/BMS) Mouse ASMs were isolated from Acta2-hrGFP;Cspg4-DsRED adult mice using flow cytometry. VAS mice were given normal, vitamin A-sufficient diet (15 IU vitamin/g) while the VAD mice were given 3 days of vitamin A-deficient diet (< 0.1 IU vitamin/g). CTR/BMS mouse ASMs were also isolated from Acta2-hrGFP;Cspg4-DsRED adult mice using flow cytometry. CTR mice were given 3 days of powdered, vitamin A sufficient diet mixed with vehicles (corn oil) while the BMS diet were given 3 days of powdered, vitamin A sufficient diet mixed with a potent pan-RA-receptor antagonist (BMS, 5 mcg/g).

2018-12-25 | GSE116846 | GEO

Human epidermal keratinocytes treated with retinoic acid or thyroid hormone

Project description:Targets of Retinoic Acid (RA) were identified in primary human epidermal keratinocytes grown in the presence or absence of all-trans retinoic acid for 1, 4, 24, 48 and 72 hours. Targets of Thyroid Hormone (T3) were identified in primary human epidermal keratinocytes grown in the presence or absence of the hormone; same controls as for RA.

2010-06-11 | GSE22298 | GEO

Human epidermal keratinocytes treated with retinoic acid or thyroid hormone

2010-06-23 | E-GEOD-22298 | biostudies-arrayexpress

Transcriptomic analysis of Retinoic acid and 9 cis retinoic acid in zebrafish.

Project description:We are presenting the application of toxicogenomics in the evaluation of the toxic effects of retinoic acid and one of its isoforms the 9-cis retinoic acid. The main goal is to distinguish the pattern of action of the both chemical compounds and their action in an extended exposure. The results suggest a different pattern within the days and the chemicals. Representatives of each GO functional groups were selected and quantified by real-time PCR to validate the microarray data and to differentiate the action of retinoic acid compounds studied.

2015-12-31 | GSE41335 | GEO

Effect of Exogenous Retinoic Acid on human iPSCs derived intestinal organoids

Project description:Retinoic acid promotes the in vitro growth, patterning and improves the cellular composition of human pluripotent stem-cell-derived intestinal organoids

2022-09-02 | GSE212333 | GEO

Global expression profiling of osteogenic differeintiation with retinoic acid or without retinoic acid for two days from hiPSC

Project description:Comparison of gene expressions among osteogenic differentiated cells with retinoic acid, those without retinoic acid and cells before induction

2018-12-21 | GSE119577 | GEO

Neural induction (Embrioid bodies-Retinoic acid)

Project description:Neural induction of ES by Retinoic acid Neural induction of ES by Retinoic acid

2010-06-11 | E-GEOD-4075 | biostudies-arrayexpress

Retinoic acid induces human gastruloids with posterior embryo-like structures

Project description:Retinoic acid induces human gastruloids with posterior embryo-like structures

| PRJNA859692 | ENA