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Transcriptomic analysis of progenitor marker expression in retinal cells

ABSTRACT: Transcriptomic analysis of progenitor marker expression in retinal cells

PROVIDER: PRJNA480689 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7511300_1.fastq.gz	Fastqsanger.gz
	SRR7511300_2.fastq.gz	Fastqsanger.gz
	SRR7511301_1.fastq.gz	Fastqsanger.gz
	SRR7511301_2.fastq.gz	Fastqsanger.gz

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Project description:The development of complex tissues requires that mitotic progenitor cells integrate information from the environment. The highly varied outcomes of such integration processes undoubtedly depend at least in part upon variations among the gene expression programs of individual progenitor cells. To date, there has not been a comprehensive examination of these differences among progenitor cells of a particular tissue. Here, we used comprehensive gene expression profiling to define these differences among individual progenitor cells of the vertebrate retina. Retinal progenitor cells (RPCs) have been shown by lineage analysis to be multipotent throughout development and to produce distinct types of daughter cells in a temporal, conserved order. A total of 42 single RPCs were profiled on Affymetrix arrays. An extensive amount of heterogeneity in gene expression among RPCs, even among cells isolated from the same developmental time point, was observed. While many classes of genes displayed heterogeneity of gene expression, the expression of transcription factors constituted a significant amount of the observed heterogeneity. Additionally, the expression of cell cycle related transcripts showed differences among those associated with G2 and M, versus G1 and S phase, suggesting different levels of regulation for these genes. These data provide insights into the types of processes and genes that are fundamental to cell fate choices, proliferation decisions, and, for cells of the central nervous system, the underpinnings of the formation of complex circuitry. Experiment Overall Design: Single retinal cells were isolated in tubes containing lysis buffer, their mRNAs were reverse transcribed, and the resulting cDNAs were PCR amplified for 35 cycles. Labeled cDNA samples were hybridized to Affymetrix 430 2.0 microarrays and the data was normalized using MAS5.0 software. A total of 42 retinal progenitors were identified post hoc and compared with ganglion, amacrine and photreceptor cells isolated from identical timepoints.

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Transcriptomic analysis of progenitor marker expression in retinal cells

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