Project description:Gene expression profile of yeast under the glycolaldehdye stress (newly discovered fermentation inhibitor) was evaluated in the experiment Two-condition design, 10 mM glycolaldehdye-treated and nontreated yeast cells, three replicates were run for each condition
Project description:Proanthocyanidins (PAs) could pose significant enhancement on the metabolism and fermentation efficiency of Saccharomyces cerevisiae yeast cells, which has been elucidated from metabolic level and cell phenotype in our previous work. The goals of this study are to compare the transcriptome profiling (RNA-seq) of yeast cells treated with and without PAs to explore the possible global protection mechanism. After treated with 0 g/L, 0.1g/L, 1.0 g/L PAs, the total RNA of Saccharomyces cerevisiae strain AWRI R2 was extracted and sequenced at Illumina HiSeq sequencing platform. The analysis yielded a total of 524025062 clean sequencing reads. The clean reads were processed for the following bioinformatics analysis.
Project description:Saccharomyces cerevisiae, SEY6210a/KMY1006a cells were treated with a sphingolipid species, phytosphingosine and time course change was monitored to understand mediator function of phytosphingosine. Gene expression differences were monitored with DNA microarray. Transcriptomic analyses showed that systemic reduction of ribosome-related genes, leading to the new insight in the transcriptional regulation downstream of phytosphingosine.