Ginkgo biloba
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA480926 | ENA |
REPOSITORIES: ENA
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Project description:Plant-derived nanovesicles (PDNVs) were isolated from Ginkgo biloba seed homogenate using differential ultracentrifugation (DUC) followed by density gradient ultracentrifugation (DGUC) with linear and non-linear iodixanol gradients. Nanoparticle tracking analysis (NTA) and cryo-transmission electron microscopy (cryo-TEM) characterized vesicle size, concentration, and morphology. Untargeted mass spectrometry profiled the protein content of distinct PDNV fractions. Ginkgo PDNVs formed a heterogeneous population, including single- and double-bilayer vesicles >50 nm. Proteomics revealed seed storage proteins (legumin, ginnacin) and membrane-associated ATPases, HSP90, catalase, PEPC, and eEF1A. Ginkgo seed-derived PDNVs exhibit promising vascular protective and anti-inflammatory properties, supporting their potential as safe, multifunctional agents for endothelial modulation.
2025-12-18 | PXD072128 | Pride
Project description:Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from different Ginkgo biloba tissues (leaves, female and male cones). The high depth of the resulting datasets enabled us to examine in detail critical small RNA features as size distribution, tissue-specific regulation and sequence conservation between different organs in this species. We also developed database resources and a dedicated website (http://smallrna.udel.edu/) with computational tools for allowing other users to identify new miRNAs or siRNAs involved in specific regulatory pathways, verify the degree of conservation of these sequences in other plant species and map small RNAs on genes or larger regions of the maize genome under study. Small RNA libraries were derived from leaves, female and male cones of Ginkgo biloba. Each tissue represented a mixture of developmental stages. Total RNA was isolated using the Plant RNA Purification Reagent (Invitrogen) and submitted to Illumina (Hayward, CA, http://www.illumina.com) for small RNA library construction using approaches described in (Lu et al., 2007) with minor modifications. The small RNA libraries were sequenced with the Sequencing-By-Synthesis (SBS) technology by Illumina. PERL scripts were designed to remove the adapter sequences and determine the abundance of each distinct small RNA. We thank Eric Brenner for providing the plant material and Kan Nobuta and Gayathri Mahalingam for assistance with the computational methods..
2011-05-09 | E-GEOD-28963 | biostudies-arrayexpress
Project description:We conducted RNA-seq from the Ginkgo leaves after UV-B treatment,and constructed the molecular regulatory network of flavonoids synthesis under UV-B radiation in G. biloba.
2019-12-13 | GSE141890 | GEO