Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Brachyspira pilosicoli 95/1000

ABSTRACT: Brachyspira pilosicoli 95/1000 genome sequencing project.

PROVIDER: PRJNA48097 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	GCA_000143725.1_sequence_report.txt	Txt

Items per page:

1 - 1 of 1

Similar Datasets

Brachyspira pilosicoli SP16

Project description:Brachyspira pilosicoli SP16 genome sequencing project

| PRJNA67877 | ENA

Brachyspira pilosicoli

Project description:Brachyspira pilosicoli overview

| PRJNA48517 | ENA

Brachyspira pilosicoli WesB

Project description:Brachyspira pilosicoli WesB Genome sequencing

| PRJEA89437 | ENA

Brachyspira pilosicoli B2904

Project description:Brachyspira pilosicoli B2904 Genome sequencing

| PRJNA80999 | ENA

Brachyspira pilosicoli

Project description:Whole genome sequencing of Brachyspira pilosicoli, an opportunistic pathogen

| PRJDB2851 | ENA

Brachyspira pilosicoli P43/6/78

Project description:Brachyspira pilosicoli P43/6/78 genome sequencing project

| PRJNA67875 | ENA

The exposed proteomes of Brachyspira hyodysenteriae and B. pilosicoli

Project description:Applying a shotgun proteomics approach, we built a unique database of Brachyspira hyodysenteriae and B. pilosicoli peptides and proteins. A total of 148 LC-MS/MS runs were conducted on an Orbitrap instrument, and more than 29000 different peptides in cell supernatants and partially digested cell suspensions of 3 strains of B. hyodysenteriae and 2 strains of B. pilosicoli were identified. Overall, 16970 and 15493 peptide sequences, which pointed to 1625 and 1338 different protein accessions, were identified in B. hyodysenteriae and B. pilosicoli, respectively. The large-scale characterization of peptides and proteins in the extracellular medium as well as of the exposed proteins on the bacterial surface in these species provides a unique collection of interrelated data on the molecules involved in the interaction of these bacteria with their environment.

2016-07-05 | PXD003900 | Pride

BPP43_05035 is a Brachyspira pilosicoli cell surface adhesin that weakens the integrity of the epithelial barrier during infection

Project description:The anaerobic spirochete Brachyspira causes intestinal spirochetosis, characterized by the intimate attachment of bacterial cells to the colonic mucosa, potentially leading to symptoms such as diarrhea, abdominal pain, and weight loss. Despite the clinical significance of Brachyspira infections, the mechanism of the interaction between Brachyspira and the colon epithelium is not known. We characterized the molecular mechanism of the B. pilosicoli-epithelium interaction and its impact on the epithelial barrier during infection. Through a proteomics approach, we identified BPP43_05035 as a candidate B. pilosicoli surface protein that mediates bacterial attachment to cultured human colonic epithelial cells. The crystal structure of BPP43_05035 revealed a globular lipoprotein with a six-bladed beta-propeller domain. Blocking the native BPP43_05035 on B. pilosicoli, either with a specific antibody or via competitive inhibition, abrogated its binding to epithelial cells, which required cell surface-exposed N-glycans. Proximity labeling and interaction assays revealed that BPP43_05035 bound to tight junctions, thereby increasing the permeability of the epithelial monolayer. Extending our investigation to humans, we discovered a downregulation of tight junction and brush border genes in B. pilosicoli-infected patients carrying detectable levels of epithelium-bound BPP43_05035. Collectively, our findings identify BPP43_05035 as a B. pilosicoli adhesin that weakens the colonic epithelial barrier during infection.

2024-10-18 | PXD056942 | Pride

Brachyspira

Project description:Brachyspira species (mostly Brachyspira pilosicoli)sequencing

| PRJNA564276 | ENA

Brachyspira

Project description:Brachyspira pilosicoli and Brachyspira hampsonii Genome sequencing and assembly

| PRJNA512878 | ENA