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Single cell RNAseq integrates T cell phenotypes associated with Listeria infection in mice

ABSTRACT: Single cell RNAseq integrates T cell phenotypes associated with Listeria infection in mice

PROVIDER: PRJNA481335 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7526010_1.fastq.gz	Fastqsanger.gz
	SRR7526010_2.fastq.gz	Fastqsanger.gz
	SRR7526011_1.fastq.gz	Fastqsanger.gz
	SRR7526011_2.fastq.gz	Fastqsanger.gz
	SRR7526012_1.fastq.gz	Fastqsanger.gz

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Single cell RNAseq integrates T cell phenotypes associated with Listeria infection in mice

Project description:Mice were inoculated with Listeria by i.p. injection on days 0 and 11. On day 18, FSC(high)CD4(+)CD8(-)B220(-)MHCII(-) T cells were FACS-sorted from spleens for single cell RNAseq.

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Listeria infection in hepatocytes

Project description:Listeria monocytogenes causes severe foodborne illness in pregnant women and immunocompromised individuals. After the intestinal phase of infection, the liver plays a central role in the clearance of this pathogen through its important functions in immunity. However, recent evidence suggests that subpopulations of L. monocytogenes may escape eradication after prolonged infection of hepatocytes, by entering a persistence phase in vacuoles. Here, we examine whether this long-term infection alters hepatocyte defense pathways, which may be instrumental for bacterial persistence. We first established models of Listeria infection in human hepatocyte cell lines HepG2 and Huh7 and in primary mouse hepatocytes (PMH). In these cells, Listeria efficiently enters the persistence stage after a 3-day infection, while inducing a type I (PMH) or type I/III (HepG2) or no (Huh7) interferon response. RNA-seq analysis identified a common signature of long-term Listeria infection on the hepatocyte transcriptome, characterized by overexpression of a set of genes involved in antiviral immunity and under-expression of many acute phase protein (APP) genes, particularly involved in the complement and coagulation systems. The decrease in APP transcript amounts correlated with lower protein abundance in the secretome of infected cells, as shown by proteomics, and also occurred in the presence of APP inducers (IL-6 or IL-1b). The results also suggest that long-term Listeria infection affects lipid metabolism pathways. Collectively, these results reveal that long-term infection with L. monocytogenes profoundly deregulates the innate immune functions of hepatocytes, which could generate an environment favorable to the establishment of persistent infection.

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ISG15 counteracts Listeria monocytogenes infection

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A p53-CCF circuit integrates genome integrity with senescence phenotypes [WGS 2]

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A p53-CCF circuit integrates genome integrity with senescence phenotypes [WGS 1]

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ISG15 counteracts Listeria monocytogenes infection

Project description:ISG15 is primarily documented as an interferon-stimulated, ubiquitin-like protein (ubl), which has anti-viral activity. Although ISG15 was the founding member of the ubl protein family, very little is known about its function. We have found that ISG15 expression in non-phagocytic cells is dramatically induced upon Listeria infection and that surprisingly this induction can be Type I Interferon independent. Listeria-mediated ISG15 induction depends on the cytosolic surveillance pathway, which senses bacterial DNA and signals through STING, TBK1, IRF3 and IRF7. Most importantly, we observed that ISG15 expression restricts Listeria infection both in vitro and in vivo. We then made use of Stable Isotope Labeling in tissue culture (SILAC) to identify the ISGylated proteins that could be responsible for the ISG15-mediated protective effect. Our SILAC analysis revealed that overexpression of ISG15 leads to a striking ISGylation of integral membrane proteins of the endoplasmic reticulum and Golgi apparatus, which correlates with increased canonical secretion of cytokines. Taken together, our data reveal a previously uncharacterized signaling pathway that restricts Listeria infection and acts via ISGylation, reinforcing the view that ISG15 is a key component of the innate immune arsenal of the mammalian host.

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ISG15 counteracts Listeria monocytogenes infection

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A p53-CCF circuit integrates genome integrity with senescence phenotypes [RNA-Seq 1]

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A p53-CCF circuit integrates genome integrity with senescence phenotypes [RNA-Seq 2]

Project description:A p53-CCF circuit integrates genome integrity with senescence phenotypes [RNA-Seq 2]

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