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Distinct microglial transcriptional responses during opioid exposure or neuropathic pain [rat]

ABSTRACT: Distinct microglial transcriptional responses during opioid exposure or neuropathic pain [rat]

PROVIDER: PRJNA481754 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7537130_1.fastq.gz	Fastqsanger.gz
	SRR7537130_2.fastq.gz	Fastqsanger.gz
	SRR7537140_1.fastq.gz	Fastqsanger.gz
	SRR7537140_2.fastq.gz	Fastqsanger.gz
	SRR7537131_1.fastq.gz	Fastqsanger.gz

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Distinct microglial transcriptional responses during opioid exposure or neuropathic pain [rat]

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Project description:Neuropathic pain is an apparently spontaneous experience triggered by abnormal physiology of the peripheral or central nervous system, which evolves with time. Neuropathic pain arising from peripheral nerve injury is characterized by a combination of spontaneous pain, hyperalgesia and allodynia. There is no evidence of this type of pain in human infants or rat pups; brachial plexus avulsion, which causes intense neuropathic pain in adults, is not painful when the injury is sustained at birth. Since infants are capable of nociception from before birth and display both acute and chronic inflammatory pain behaviour from an early neonatal age, it appears that the mechanisms underlying neuropathic pain are differentially regulated over a prolonged postnatal period. We used microarrays to detail the global programme of gene expression underlying the differences in nerve injury between along the postnatal development and identified distinct classes of regulated genes during the injury Experiment Overall Design: We have performed a microarray analysis of the rat L4/L5 dorsal root ganglia, 7 days post spared nerve injury, a model of neuropathic pain. Genes that are regulated in adult rats displaying neuropathic behaviour were compared to those regulated in young rats (10 days old) that did not show the same neuropathic behaviour.

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