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Targets of DNA binding, N-terminal, or C-terminal domain swapped mutant forms fo Foxa proteins in induced hepatocyte-like (iHep) cells.

ABSTRACT: Targets of DNA binding, N-terminal, or C-terminal domain swapped mutant forms fo Foxa proteins in induced hepatocyte-like (iHep) cells.

PROVIDER: PRJNA481781 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10901261.fastq.gz	Fastqsanger.gz
	SRR10901262.fastq.gz	Fastqsanger.gz
	SRR7537870.fastq.gz	Fastqsanger.gz
	SRR7537880.fastq.gz	Fastqsanger.gz
	SRR7537871.fastq.gz	Fastqsanger.gz

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Targets of DNA binding, N-terminal, or C-terminal domain swapped mutant forms fo Foxa proteins in induced hepatocyte-like (iHep) cells.

Project description:We have found that three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro, and DNA binding, N-terminal, and C-terminal domains (DBD, NTD, and CTD, respectively) of Foxa proteins are swappable in the direct reprogramming of the iHep cells. Then we used ChIP-seq to explore the targets of the domain swapped mutant forms of Foxa proteins during conversion event to iHep cells.

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miRNA expression profile of follicular (FO) and marginal zone (MZ) B cells

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Project description:We have analyzed miRNA expression profile in FO and MZ B cells to identified differentially expressed miRNAs between this two subsets that could be potencially involved in the regulation of terminal B cell differentiation Spleens from CD19-Creki/+Dicerfl/+ and CD19-Creki/+Dicerfl/fl mice were collected and after erythrocyte lysis, splenocytes were stained with anti-B220, anti-CD21 and anti-CD23 antibodies. B220+CD21brightCD23+ (MZ) and B220+CD21+CD23bright (FO) B cell populations were sorted and total RNA of purified populations was extracted with TRIzol. miRNA microarray hybridations were performed on Mouse miRNA Microarray platform (Agilent Technologies).

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Global gene expression analysis for mouse embryonic fibroblasts (MEFs), induced hepatocyte-like (iHep) cells and adult mouse hepatocytes.

Project description:In order to characterize iHep cells more precisely, we conducted global gene expression analyses using microarrays to compare among the gene expression profiles of MEFs, iHep cells and adult mouse hepatocytes. Three MEF samples that were individually prepared from 13.5 days post coitum (dpc) embryos, three types of iHep cells and hepatocytes obtained from the adult mouse liver were used.

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