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Expression data from eosinophils isolated from subcutaneous fat of WT and KLF3 KO mice

ABSTRACT: Expression data from eosinophils isolated from subcutaneous fat of WT and KLF3 KO mice

PROVIDER: PRJNA482126 | ENA |

REPOSITORIES: ENA

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Expression data from eosinophils isolated from subcutaneous fat of WT and KLF3 KO mice

Project description:Gene expression in eosinophils isolated by FACS from inguinal subcutaneous adipose tissue of WT and mice lacking the transcriptional repressor Kruppel-like factor 3 (KLF3). We aimed to investigate deregulation of pathways and genes in the absence of KLF3 in eosinophils that reside in the subcutaneous adipose tissue, in order to study the role of KLF3 and its target genes in adipose eosinophil function and activity.

2019-04-30 | GSE117445 | GEO

Gene expression data from WT and Klf3 KO mouse CD19+ splenic B cells

Project description:To investigate the roles of Klf3 in B lymphopoiesis, CD19+ B cells were sorted from the spleens of WT and Klf3 KO mice (Molecular and Cellular Biology (2008); 28:3967–3978). Following RNA extraction, gene expression was compared in WT and Klf3 KO CD19+splenic B cells using Affymetrix microarrays.

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Gene expression data from WT and Klf3 KO mouse CD19+ splenic B cells

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Identification of candidate genes that specifically regulate subcutaneous and intramuscular fat deposition from transcriptomic and proteomic profiles in Dingyuan pigs

Project description:we collected tissues of subcutaneous fat and longissimus dorsi (LD) muscle from individuals that have divergent of backfat thickness and intramuscular fat content, and have similar age and body weight. The transcriptomic and proteomic data were gained using RNA-Seq and TMT to identify the key genes and pathways that specifically regulate the subcutaneous fat and intramuscular fat deposition in Dingyuan pig.

2022-04-04 | PXD027061 | Pride

Microarray analysis of wildtype and Klf3 KO E14.5 fetal liver cells (TER119- and TER119+)

Project description:The aim of this experiment was to investigate the role of KLF3 in regulating gene expression at different stages throughout the erythroid maturation process. Affymetrix microarrays were performed on fetal liver cells (both TER119- progenitor cells and TER119+ erythroblast cells) from E14.5 wildtype and Klf3 KO mice. Four wildtype TER119- replicates, four Klf3 KO TER119- replicates, four wildtype TER119+ replicates, three Klf3 KO TER119+ replicates. All are from E14.5 fetal liver.

2012-02-07 | E-GEOD-35592 | biostudies-arrayexpress

Transcriptomic profiling of blood and adipose-resident eosinophils from mice

Project description:Eosinophils are immune effector cells with beneficial homeostatic roles in fat. Here, bulk RNA-seq profiling revealed distinct transcriptomic signatures in fat-resident eosinophils and circulating eosinophils.

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Microarray analysis of wildtype, Klf3 H275R homozygous and heterozygous, Klf3 CH gene trap homozygous and heterozygous E12.5 embryos

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Expression data from WT and Klf3 KO BMDMs treated with LPS

Project description:Gene expression in bone marrow-derived macrophages (BMDMs) from WT and mice lacking the transcriptional repressor Kruppel-like factor 3 (KLF3). We cultured BMDMs from bone marrow for 7-10 days then treated cells with 100 ng/mL lipopolysaccharide (LPS) or vehicle (PBS) for 0 h or 8 h, followed by RNA extraction. We aimed to investigate deregulated genes and pathways in macrophages lacking KLF3, during the inflammatory response to endotoxin (LPS).

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Microarray analysis of wildtype and Klf3 KO E14.5 fetal liver cells (TER119- and TER119+)

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2013-01-31 | E-GEOD-43908 | biostudies-arrayexpress

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