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Genome-wide maps of chromatin state in renin cells [ATAC-Seq]

ABSTRACT: Genome-wide maps of chromatin state in renin cells [ATAC-Seq]

PROVIDER: PRJNA483025 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7608400_1.fastq.gz	Fastqsanger.gz
	SRR7608400_2.fastq.gz	Fastqsanger.gz
	SRR7608401_1.fastq.gz	Fastqsanger.gz
	SRR7608401_2.fastq.gz	Fastqsanger.gz
	SRR7608402_1.fastq.gz	Fastqsanger.gz

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Genome-wide maps of chromatin state in renin cells [ChIP-Seq]

Project description:We generated genome-wide chromatin-state maps of mouse renin cells: chronically recruited cells and As4.1 cell line. We found that H3K27ac can discriminate a set of regulatory regions unique for the renin cells.

2020-10-31 | GSE117722 | GEO

Genome-wide maps of chromatin state in renin cells

Project description:Genome-wide maps of chromatin state in renin cells

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Genome-wide maps of chromatin state in renin cells [ChIP-Seq]

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Genome-wide maps of chromatin state in renin cells [ATAC-Seq]

Project description:ATAC-seq was performed to identify open chromatin regions in juxtaglomerular cells, cells isolated from acutely and chronically recruited mice and in As4.1 cells

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Renin Regulatory Pathway Inhibition Epigenetically Silences *Ren1* (ATAC-Seq)

Project description:Background: Renin-expressing cells are myoendocrine cells crucial for survival which detect changes in blood pressure and release renin to maintain homeostasis. Renin is regulated through several pathways including cAMP, p300/CBP, and BET proteins including Brd4. Binding to the cAMP-responsive element in the renin enhancer region amplifies renin transcription and relies on each of these factors for an appropriate response. The specific regulatory changes that occur following inhibition of these pathways remains understudied. Therefore, we aimed to evaluate chromatin changes and gene signatures that occur following inhibition of renin regulatory factors. Methods: We treated As4.1 cells (a tumoral cell line that constitutively expresses renin) with three inhibitors (H89: PKA inhibition; JQ1: Brd4 inhibition; A-485: p300/CBP inhibition) that target required factors for renin transcriptional regulation. We then performed ATAC-seq, scRNA-seq, and ChIP-seq for H3K27ac and P300 binding on biological replicates of treated and control As4.1 cells. Results: Ren1 expression is significantly, but transiently, reduced following each inhibitory treatment. Further, PKA inhibition leads to corresponding losses in H3K27ac and p300 binding at the locus. A restricted set of nine genes with overlapping dynamically accessible regions, differential gene expression, and H3K27ac and p300 binding were identified with roles across three primary renin regulatory paradigms. Conclusions: The data shows that renin expression is regulated through a switch from an active to poised state of epigenetic control, a shift toward a less differentiated cellular identity via the loss of enhancer elements, and the disruption of cAMP, baroreceptor, and Notch mediated renin regulatory pathways.

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Project description:We generated genome-wide chromatin state and RNA Polymerase II binding maps in mouse erythroid cells by ChIP-Seq. Examination of 4 different histone modifications (H3K4me3, H3K4me1, H3K27me3, H3K27ac) and RNA Polymerase II (RNAP2) binding in mouse erythroid cells (Ter119+).

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