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RNA-Seq As4.1 cell line and native renin cells isolated from Ren1c-YFP mice

ABSTRACT: RNA-Seq As4.1 cell line and native renin cells isolated from Ren1c-YFP mice

PROVIDER: PRJNA483026 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7608420_1.fastq.gz	Fastqsanger.gz
	SRR7608420_2.fastq.gz	Fastqsanger.gz
	SRR7608415_1.fastq.gz	Fastqsanger.gz
	SRR7608415_2.fastq.gz	Fastqsanger.gz
	SRR7608416_1.fastq.gz	Fastqsanger.gz

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RNA-Seq As4.1 cell line and native renin cells isolated from Ren1c-YFP mice

Project description:RNA-seq was performed to compare the transcriptome of three renin cell types: juxtaglomerular cells, cells isolated from chronically recruited mice and in As4.1 cells

2020-10-31 | GSE117723 | GEO

RNA-seq Renin cells isolated from Ren1c-YFP mice with aortic coarctation

Project description:RNA-seq Renin cells isolated from Ren1c-YFP mice with aortic coarctation

| PRJNA662486 | ENA

Gene expression profiles of renin producing cells in the adult kidney (captopril treated animals) isolated from Renin-YFP transgenic mice using FACS (GUDMAP Series ID:46).

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Renin-YFP transgenic mice were utilized to isolate renin producing cells from the kidneys of Adult mice treated with captopril, which was provided in the drinking water at a concentration of 0.5mg/ml. In addition, mice were given low-salt chow. Mice were treated for two weeks before renin producing cells were isolated from the kidney by fluorescent activated cell sorting (FACS). RNA was isolated from FACS sorted cells and the gene expression profiles were determined by microarrays.

2012-12-05 | E-GEOD-42712 | biostudies-arrayexpress

RNA-seq Renin cells isolated from Ren1c-YFP mice with aortic coarctation

Project description:RNA-seq was performed to compare the transcriptome of renin cells to test the effects of renal perfusion pressure changes

2021-05-26 | GSE157699 | GEO

YFP+PDGFRa+ and YFP+PDGFRa– stromal cells isolated from the small intestine of LTBRfloxedPDGFRa/YFP mice

Project description:YFP+PDGFRa+ and YFP+PDGFRa– stromal cells isolated from the small intestine of LTBRfloxedPDGFRa/YFP mice

| PRJNA824320 | ENA

YFP+ and YFP– stromal cells isolated from the small intestine of LTBRYFP mice

Project description:YFP+ and YFP– stromal cells isolated from the small intestine of LTBRYFP mice

| PRJNA824321 | ENA

YFP+ and YFP– stromal cells isolated from intestinal ulcers of indomethacin treated LTBRYFP mice

Project description:YFP+ and YFP– stromal cells isolated from intestinal ulcers of indomethacin treated LTBRYFP mice

| PRJNA824319 | ENA

Genome-wide maps of chromatin state in renin cells [ChIP-Seq]

Project description:We generated genome-wide chromatin-state maps of mouse renin cells: chronically recruited cells and As4.1 cell line. We found that H3K27ac can discriminate a set of regulatory regions unique for the renin cells.

2020-10-31 | GSE117722 | GEO

Renin Regulatory Pathway Inhibition Epigenetically Silences *Ren1* (CUTnTag)

Project description:Background: Renin-expressing cells are myoendocrine cells crucial for survival which detect changes in blood pressure and release renin to maintain homeostasis. Renin is regulated through several pathways including cAMP, p300/CBP, and BET proteins including Brd4. Binding to the cAMP-responsive element in the renin enhancer region amplifies renin transcription and relies on each of these factors for an appropriate response. The specific regulatory changes that occur following inhibition of these pathways remains understudied. Therefore, we aimed to evaluate chromatin changes and gene signatures that occur following inhibition of renin regulatory factors. Methods: We treated As4.1 cells (a tumoral cell line that constitutively expresses renin) with three inhibitors (H89: PKA inhibition; JQ1: Brd4 inhibition; A-485: p300/CBP inhibition) that target required factors for renin transcriptional regulation. We then performed ATAC-seq, scRNA-seq, and ChIP-seq for H3K27ac and P300 binding on biological replicates of treated and control As4.1 cells. Results: Ren1 expression is significantly, but transiently, reduced following each inhibitory treatment. Further, PKA inhibition leads to corresponding losses in H3K27ac and p300 binding at the locus. A restricted set of nine genes with overlapping dynamically accessible regions, differential gene expression, and H3K27ac and p300 binding were identified with roles across three primary renin regulatory paradigms. Conclusions: The data shows that renin expression is regulated through a switch from an active to poised state of epigenetic control, a shift toward a less differentiated cellular identity via the loss of enhancer elements, and the disruption of cAMP, baroreceptor, and Notch mediated renin regulatory pathways.

2024-08-26 | GSE254943 | GEO

Smart-seq2 analysis of P17 FACS sorted retinal cells from the Kcng4-cre;stop-YFP X Thy1-stop-YFP Line#1 mice

Project description:Four Kcng4-cre;stop-YFP mouse retinas from two mice were dissected, dissociated and FACS sorted, and single cell RNA-seq libraries were generated for 384 single cells using Smart-seq2. Aligned bam files are generated for 383 samples as one failed to align. Four mouse retinas (labeled 1la, 1Ra, and 2la, 2Ra respective from the two mice) were used, and 96 single cells from each were processed using Smart-seq2. Total 384 cells Smart-seq2 analysis of P17 FACS sorted retinal cells from the Kcng4-cre;stop-YFP mice (Kcng4tm1.1(cre)Jrs mice [Duan et al., Cell 158, 793-807, 2015] crossed to the cre-dependent reporter Thy1-stop-YFP Line#1 [Buffelli et al., Nature 424, 430-434, 2003])

2016-08-25 | E-GEOD-81903 | biostudies-arrayexpress

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